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Multi environment evaluation of chickpea disease nurseries (wilt, Aschochyta blight and Botrytis grey mold) to identify stable and new sources of resistance
Under the ICRISAT- ICAR collaboration, disease nurseries of wilt (ICWN), AB (IABN) and BGM (IBGMN) consisting of 30 entries (28 resistant cultivars and 2 susceptible checks) were evaluated at different locations (hot spots) ...
An Update on Genetic Resistance of Chickpea to Ascochyta Blight
Ascochyta blight (AB) caused by Ascochyta rabiei (Pass.) Labr. is an important and
widespread disease of chickpea (Cicer arietinum L.) worldwide. The disease is particularly severe
under cool and humid weather conditions. ...
Quantification of Foc colonization through qPCR in resistant and susceptible cultivars grown under elevated CO2
The chickpea seedlings of susceptible cv. JG 62 and resistant cv. WR 315 were grown in open top chambers at different CO2 concentrations [ambient (~380 ppm) and elevated ( 550 ppm and 700 ppm). Seven day old seedlings were ...
Multi-environment evaluation of pigeonpea disease nursery of 28 lines to wilt and SMD resistance
Under the ICAR-ICRISAT collaboration, during 2015-16 crop seasons, Pigeonpea Wilt and SM Disease Nursery (PWSMDN) consisting of 30 entries (28 resistant cultivars and 2 susceptible checks) were evaluated for wilt and SMD ...
Development of proteome library from FOC inoculated chickpea resistant and susceptible cultivars under elevated CO2 through MALDI and MS-MS analysis
Total root proteins of control and Foc infected chickpea (JG 62 and WR 315) were extracted. Isoelectric focusing (IEF) of extracted proteins was carried out using immobilized pH gradient (IPG) strips. 2-DE gels were scanned ...
Application of Modern Tools of Biotechnology for Pest Management
Recombinant DNA technology has significantly enhanced our ability for crop improvement, to meet the increased demand for food and fiber. Considerable progress has been made over the past two decades in manipulating genes ...
Expression profiling from different tissues and developmental stages to define gene atlas
Thirty seven different samples representing 19 aerial, seven underground, two germinal and nine seed development related tissues from five different developmental stages have been targeted. The five developmental stages ...
Identification and Validation of Reference Genes and Their Impact on Normalized Gene Expression Studies across Cultivated and Wild Cicer Species
Quantitative Real-Time PCR (qPCR) is a preferred and reliable method for accurate quantification
of gene expression to understand precise gene functions. A total of 25 candidate
reference genes including traditional and ...
Geographical distribution of traits and diversity in the world collection of pearl millet [Pennisetum glaucum (L.) R. Br., synonym: Cenchrus americanus (L.) Morrone] landraces conserved at the ICRISAT genebank
The genebank at ICRISAT conserves the
largest collection of 23,092 pearl millet germplasm
accessions originating in 52 countries. A total of 15,979
landraces originating in 34 countries and having geographic
coordinates ...
Gene action and combining ability estimates using cytoplasmic-genic male sterile lines to develop pigeonpea hybrids for rainfed condition
Pigeonpea is the major source of vegetable protein in
Indian diet. About 72.5 percent area and 62.5 production of
world’s pigeonpea is in India.Pigeonpea is the only food legume
where cytoplasmic-genic male sterility is ...