Population structure analysis and determination of neurotoxin content in grass pea
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Grass pea (Lathyrus sativus L.) is considered as one of the resilient crops for adaptation to climate change. Due to protein-rich seeds and leaves it has strong potential as human food as well as animal feed and fodder. However, genetic improvement in this crop remains stagnant due to the poorly characterized genetic resources. In this study, we characterized 118 accessions of grass pea with 18 EST-SSR markers. Among the test accessions, 100 represented cultivated accessions (Lathyrus sativus L.) and 18 wild accessions belonging to 17 Lathyrus species. A total of 67 alleles were detected with average of 3.72 alleles per locus and average polymorphism information content (PIC) value of 0.52. Hierarchical cluster analysis using UPGMA method grouped genotypes into four main clusters. The STRUCTURE (version 2.3.4) analysis indicated significant population structure in grass pea. The entire population identified four genetically distinct groups. Group I consisted of 34 accessions with low β-N-Oxalyl-L-α, β-diamino propionic acid (β-ODAP) concentration. Only one accession was wild type (L. cicera) in Group I. Group II was represented by 19 accessions. Group II included 17 wild accessions along with two cultivated accessions (local landraces), namely, Bang-9 (IG114506) and Bang-11 (IG114509). Group III had 36 accessions of cultivated species with both low and high β-ODAP concentration. Group IV consisted of 29 accessions of cultivated species with both high and low β-ODAP concentration. Variation in grass pea accessions was 17% among populations and 83% within populations. Seed ODAP concentration ranged from 0.13-0.57 per cent while testing 100 Lathyrus genotypes from Bangladesh. These characterized accessions will be useful in grass pea breeding program.
- ICARDA