| dc.contributor | Li, Bei | en_US |
| dc.contributor | Pandey, Manish K | en_US |
| dc.contributor | Wu, Yanshan | en_US |
| dc.contributor | Lei, Yong | en_US |
| dc.contributor | Yan, Liying | en_US |
| dc.contributor | Dai, Xiaofeng | en_US |
| dc.contributor | Jiang, Huifang | en_US |
| dc.contributor | Zhang, Juncheng | en_US |
| dc.contributor | Wei, Guo | en_US |
| dc.contributor | Varshney, Rajeev | en_US |
| dc.contributor | Liao, Boshou | en_US |
| dc.creator | wan, liyun | en_US |
| dc.date.accessioned | 2017-04-26T21:15:38Z | |
| dc.date.available | 2017-04-26T21:15:38Z | |
| dc.identifier | http://oar.icrisat.org/id/eprint/9838 | en_US |
| dc.identifier | https://mel.cgiar.org/reporting/download/hash/w2dtVnfX | en_US |
| dc.identifier.citation | Liyun wan, Bei Li, Manish K Pandey, Yanshan Wu, Yong Lei, Liying Yan, Xiaofeng Dai, Huifang Jiang, Juncheng Zhang, Guo Wei, Rajeev Varshney, Boshou Liao. (31/10/2016). Transcriptome Analysis of a New Peanut Seed Coat Mutant for the Physiological Regulatory Mechanism Involved in Seed Coat Cracking and Pigmentation. Frontiers in Plant Science, 7 (1491), pp. 1-15. | en_US |
| dc.identifier.uri | https://hdl.handle.net/20.500.11766/6892 | |
| dc.description.abstract | Seed-coatcrackingandundesirablecolorofseedcoathighlyaffectsexternalappearanceandcommercialvalueofpeanuts(ArachishypogaeaL.).Withanobjectivetofindgeneticsolutiontotheaboveproblems,apeanutmutantwithcrackingandbrowncoloredseedcoat(testa)wasidentifiedfromanEMStreatedmutantpopulationanddesignatedas“peanutseedcoatcrackandbrowncolormutantline(pscb).”Theseedcoatweightofthemutantwasalmosttwiceofthewildtype,andthegerminationtimewassignificantlyshorterthanwildtype.Further,themutanthadlowerleveloflignin,anthocyanin,proanthocyanidincontent,andhighlyincreasedlevelofmelanincontentascomparedtowildtype.UsingRNA-Seq,weexaminedtheseedcoattranscriptomeinthreestagesofseeddevelopmentinthewildtypeandthepscbmutant.TheRNA-Seqanalysisrevealedpresenceofhighlydifferentiallyexpressedphenylpropanoidandflavonoidpathwaygenesinallthethreeseeddevelopmentstges,especiallyat40daysafterflowering(DAF40).Also,theexpressionofpolyphenoloxidasesandperoxidasewerefoundtobeactivatedsignificantlyespeciallyinthelateseeddevelopmentalstage.Thegenome-widecomparativestudyoftheexpressionprofilesrevealed62differentiallyexpressedgenescommonacrossallthethreestages.Byanalyzingtheexpressionpatternsandthesequencesofthecommondifferentiallyexpressedgenesofthethreestages,threecandidategenesnamelyc36498_g1(CCoAOMT1),c40902_g2(kinesin),andc33560_g1(MYB3)wereidentifiedresponsibleforseed-coatcrackingandbrowncolorphenotype.Therefore,thisstudynotonlyprovidedcandidategenesbutalsoprovidedgreaterinsightsandmoleculargeneticcontrolofpeanutseed-coatcrackingandcolorvariation.Theinformationgeneratedinthisstudywillfacilitatefurtheridentificationofcausalgeneanddiagnosticmarkersforbreedingimprovedpeanutvrietieswithsmoothanddesirableseedcoatcolor. | en_US |
| dc.format | PDF | en_US |
| dc.language | en | en_US |
| dc.publisher | Frontiers Media | en_US |
| dc.rights | CC-BY-NC-4.0 | en_US |
| dc.source | Frontiers in Plant Science;7 (1491),(2016) Pagination 1,15 | en_US |
| dc.subject | rna-seq | en_US |
| dc.subject | peanut (arachishypogaea) | en_US |
| dc.subject | seed-coatcracking | en_US |
| dc.subject | flavonoidpathway | en_US |
| dc.subject | Peanut | en_US |
| dc.title | Transcriptome Analysis of a New Peanut Seed Coat Mutant for the Physiological Regulatory Mechanism Involved in Seed Coat Cracking and Pigmentation | en_US |
| dc.type | Journal Article | en_US |
| dcterms.available | 2016-10-31 | en_US |
| dcterms.extent | 1-15 | en_US |
| cg.creator.id | Pandey, Manish K: 0000-0002-4101-6530 | en_US |
| cg.subject.agrovoc | agriculture | en_US |
| cg.subject.agrovoc | pigmentation | en_US |
| cg.contributor.center | Chinese Academy of Agricultural Sciences, Oil Crops Research Institute - CAAS-OCRI | en_US |
| cg.contributor.center | International Crops Research Institute for the Semi-Arid Tropics - ICRISAT | en_US |
| cg.contributor.center | Chinese Academy of Agricultural Sciences, Institiute of Food Sciencea and Technology - CAAS-FOODCAAS | en_US |
| cg.contributor.crp | CGIAR Research Program on Grain Legumes - GL | en_US |
| cg.contributor.funder | Not Applicable | en_US |
| cg.coverage.region | Eastern Asia | en_US |
| cg.coverage.region | Southern Asia | en_US |
| cg.coverage.country | CN | en_US |
| cg.coverage.country | IN | en_US |
| cg.contact | lboshou@hotmail.com | en_US |
| cg.identifier.doi | https://dx.doi.org/10.3389/fpls.2016.01491 | en_US |
| cg.isijournal | ISI journal | en_US |
| dc.identifier.status | Open access | en_US |
| mel.impact-factor | 4.495 | en_US |
| cg.issn | ESSN: 1664-462X | en_US |
| cg.journal | Frontiers in Plant Science | en_US |
| cg.volume | 7 (1491) | en_US |
