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dc.contributorAl-Debei, Hmouden_US
dc.contributorAyad, Jamal Yousefen_US
dc.contributorHasan, Shireenen_US
dc.creatorAl-Abdallat, Ayeden_US
dc.date2014-10-28en_US
dc.date.accessioned2017-07-24T01:48:23Z
dc.date.available2017-07-24T01:48:23Z
dc.identifierhttp://dx.doi.org/10.3390/ijms151119499en_US
dc.identifierhttps://mel.cgiar.org/reporting/download/hash/lwjiKDBKen_US
dc.identifier.citationAyed Al-Abdallat, Hmoud Al-Debei, Jamal Yousef Ayad, Shireen Hasan. (28/10/2014). Over-Expression of SlSHN1 Gene Improves Drought Tolerance by Increasing Cuticular Wax Accumulation in Tomato. International Journal of Molecular Sciences, 15, pp. 19499-19515;.en_US
dc.identifier.urihttp://hdl.handle.net/20.500.11766/7342
dc.description.abstractIncreasing cuticular wax accumulation in plants has been associated with improving drought tolerance in plants. In this study, a cDNA clone encoding the SlSHN1 transcription factor, the closest ortholog to WIN/SHN1 gene in Arabidopsis, was isolated from tomato plant. Expression analysis of SlSHN1 indicated that it is induced in response to drought conditions. The over-expression of SlSHN1 in tomato under the control of the constitutive CaMV 35S promoter produced plants that showed mild growth retardation phenotype with shiny and dark green leaves. Scanning electron microscopy showed that the over-expression of SlSHN1 in tomato resulted in higher cuticular wax deposition on leaf epidermial tissue when compared to non-transformed plants. Expression analysis in transgenic lines over-expressing SlSHN1 indicated that several wax-related synthesis genes were induced. Transgenic tomato plants over-expressing SlSHN1 showed higher drought tolerance when compared with wild type plants; this was reflected in delayed wilting of transgenic lines, improved water status and reduced water loss rate when compared with wild type plants. In conclusion, the SlSHN1 gene can modulate wax accumulation and could be utilized to enhance drought tolerance in tomato planten_US
dc.formatPDFen_US
dc.languageenen_US
dc.publisherMDPIen_US
dc.rightsBY-NCen_US
dc.sourceInternational Journal of Molecular Sciences;15,(2014) Pagination 19499,19515;en_US
dc.subjectcuticleen_US
dc.subjectsolanum lycopersicumen_US
dc.subjecttranscription factoren_US
dc.subjectTomatoen_US
dc.titleOver-Expression of SlSHN1 Gene Improves Drought Tolerance by Increasing Cuticular Wax Accumulation in Tomatoen_US
dc.typeJournal Articleen_US
cg.subject.agrovocdroughten_US
cg.subject.agrovocbioinformaticsen_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.centerUniversity of Jordan - JUen_US
cg.contributor.crpCRP on Dryland Systems - DSen_US
cg.contributor.funderInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.projectCommunication and Documentation Information Services (CODIS)en_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.coverage.regionGlobalen_US
cg.contactA.Al-Abdallat@cgiar.orgen_US
dc.identifier.statusOpen accessen_US
mel.project.openhttps://mel.cgiar.org/projects/codisen_US


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