dc.contributor | Jaganathan, Deepa | en_US |
dc.contributor | Kudapa, Hima bindu | en_US |
dc.contributor | Thudi, Mahendar | en_US |
dc.contributor | Roorkiwal, Manish | en_US |
dc.contributor | Katta, Mohan AVS | en_US |
dc.contributor | Doddamani, Dadakhalandar | en_US |
dc.contributor | Vanika, Garg | en_US |
dc.contributor | Kavi Kishor, Polavarapu B. | en_US |
dc.contributor | Gaur, Pooran | en_US |
dc.contributor | Nguyen, Henry T. | en_US |
dc.contributor | Batley, Jacqueline | en_US |
dc.contributor | Edwards, David | en_US |
dc.contributor | Sutton, Tim | en_US |
dc.contributor | Varshney, Rajeev | en_US |
dc.creator | Kale, Sandip | en_US |
dc.date.accessioned | 2017-08-16T11:49:13Z | |
dc.date.available | 2017-08-16T11:49:13Z | |
dc.identifier | https://mel.cgiar.org/dspace/limited | en_US |
dc.identifier | http://oar.icrisat.org/9136/1/srep15296.pdf | en_US |
dc.identifier.citation | Sandip Kale, Deepa Jaganathan, Hima bindu Kudapa, Mahendar Thudi, Manish Roorkiwal, Mohan AVS Katta, Dadakhalandar Doddamani, Garg Vanika, Polavarapu B. Kavi Kishor, Pooran Gaur, Henry T. Nguyen, Jacqueline Batley, David Edwards, Tim Sutton, Rajeev Varshney. (19/10/2015). Prioritization of candidate genes in “QTL-hotspot” region for drought tolerance in chickpea (Cicer arietinum L. ). Scientific Reports, 5 (15296). | en_US |
dc.identifier.uri | https://hdl.handle.net/20.500.11766/7433 | |
dc.description.abstract | A combination of two approaches, namely QTL analysis and gene enrichment analysis were used
to identify candidate genes in the “QTL-hotspot” region for drought tolerance present on the Ca4
pseudomolecule in chickpea. In the first approach, a high-density bin map was developed using
53,223 single nucleotide polymorphisms (SNPs) identified in the recombinant inbred line (RIL)
population of ICC 4958 (drought tolerant) and ICC 1882 (drought sensitive) cross. QTL analysis using
recombination bins as markers along with the phenotyping data for 17 drought tolerance related
traits obtained over 1–5 seasons and 1–5 locations split the “QTL-hotspot” region into two subregions
namely “QTL-hotspot_a” (15 genes) and “QTL-hotspot_b” (11 genes). In the second approach,
gene enrichment analysis using significant marker trait associations based on SNPs from the Ca4
pseudomolecule with the above mentioned phenotyping data, and the candidate genes from the
refined “QTL-hotspot” region showed enrichment for 23 genes. Twelve genes were found common
in both approaches. Functional validation using quantitative real-time PCR (qRT-PCR) indicated four
promising candidate genes having functional implications on the effect of “QTL-hotspot” for drought
tolerance in chickpea. | en_US |
dc.format | PDF | en_US |
dc.language | en | en_US |
dc.publisher | Nature Publishing Group | en_US |
dc.source | Scientific Reports;5,(2015) | en_US |
dc.subject | qtl-hotspot | en_US |
dc.title | Prioritization of candidate genes in “QTL-hotspot” region for drought tolerance in chickpea (Cicer arietinum L.) | en_US |
dc.type | Journal Article | en_US |
dcterms.available | 2015-10-19 | en_US |
cg.creator.id | Thudi, Mahendar: 0000-0003-2851-6837 | en_US |
cg.creator.id | Roorkiwal, Manish: 0000-0001-6595-281X | en_US |
cg.subject.agrovoc | plant genetic resources | en_US |
cg.subject.agrovoc | drought tolerance | en_US |
cg.subject.agrovoc | Chickpea | en_US |
cg.contributor.center | International Crops Research Institute for the Semi-Arid Tropics - ICRISAT | en_US |
cg.contributor.center | The University of Western Australia, Faculty of Science, School of Plant Biology - UWA - FoS - SoPB | en_US |
cg.contributor.center | University of Adelaide - Adelaide | en_US |
cg.contributor.center | Osmania University | en_US |
cg.contributor.center | University of Missouri - MU USA | en_US |
cg.contributor.crp | CGIAR Research Program on Grain Legumes - GL | en_US |
cg.contributor.funder | Not Applicable | en_US |
cg.contributor.project-lead-institute | International Crops Research Institute for the Semi-Arid Tropics - ICRISAT | en_US |
cg.date.embargo-end-date | 2025-12-31 | en_US |
cg.contact | S.Kale@cgiar.org | en_US |
cg.identifier.doi | https://dx.doi.org/10.1038/srep15296 | en_US |
cg.isijournal | ISI Journal | en_US |
dc.identifier.status | Limited access | en_US |
mel.impact-factor | 5,15296 | en_US |
cg.journal | Scientific Reports | en_US |
cg.issue | 15296 | en_US |
cg.volume | 5 | en_US |