Efficient Production of Tetraploid Barley (Hordeum vulgare L.) by Colchicine Treatment of Diploid Barley
An experiment was conducted to induce tetraploidy in three diploid barley varieties (Martin, Rihane and Manel) through different colchicines treatments. Colchicine was added for three different concentrations at three different stages of plant development i.e. on seed (0.05% for 48 hours), on pre-germinated seeds (0.1% for 2 hours) and on three leaf stage (0.1% for 16 hours). Colchicine application reduced significantly germination percentage and viability of plants. Seed germination was completely inhibited in Martin, while a reduction of 20% and 30% for germination percentage compared to control was recorded in varieties Manel and Rihane, respectively at 0.1% colchicine concentration. Ploidy evaluation showed no tetraploidy in all the three tested varieties by colchicine application of 0.05% for 48 hours on seeds and 0.1% for 2 hours on pre-germinated seeds. However, tetraploid plants were produced only by treatment with 0.1% for 16 hours of seedlings. The percentages of plants were 40%, 44% and 100% for Rihane, Manel and Martin, respectively. Cytological analyses showed the increase of chromosome numbers from 2n=2x=14 to 2n=4x=28. The increase of ploidy levels caused major changes in some morphological traits. In fact, the induced tetraploids in barley was accompanied by significant (P<0.01) decrease in plant height, tiller height, leaf number and leaf length compared to diploid control plants. colchicine treatment induce successfully the production of tetraploid barley plants and could be used in breeding programs.
- ICARDA 
Showing items related by title, author, creator and subject.
Title: ICARDA and EIAR Success of Joint Barley-Breeding Program in Developing Food Barley Varieties for the Ethiopian Low-Moisture Areas Author: Setotaw, Tesfahun AlemuDate: 2012-11-27Type: Journal ArticleStatus: Open accessCollaborative work of international research institutes, like ICARDA and national agricultural research centers in any developing country, such as Ethiopia, is important and has significant impact on improving the livelihood ...
Title: Variation at the vernalisation genes Vrn‑H1 and Vrn‑H2 determines growth and yield stability in barley (Hordeum vulgare) grown under dryland conditions in Syria Author: Rollins, Jarod; 0000-0002-8248-6088; ORCIDDate: 2013-11-27Type: Journal ArticleStatus: Open accessThe objective of the present study was to identify QTL influencing agronomic performance in rain-fed Mediterranean environments in a recombinant inbred line (RIL) population, ARKE derived from the Syrian barley landrace, ...
Title: Agronomic Performance and Quality Properties of Malting Barley Genotypes Assessed Rain Fed in Bekaa Province, Lebanon Author: Chalak, LamisDate: 2015-06-27Type: Journal ArticleStatus: Open accessThe use of barley in Lebanon is still limited to feed despite the increased beer consumption and the amplified demand by local industries of imported malt. In the present work, the performance of 11 foreign malting genotypes ...