Show simple item record

dc.contributorPanis, Bartholomeusen_US
dc.creatorFemke, Smetsen_US
dc.date2016-05-30en_US
dc.date.accessioned2018-04-16T00:15:52Z
dc.date.available2018-04-16T00:15:52Z
dc.identifierhttps://mel.cgiar.org/dspace/limiteden_US
dc.identifier.citationSmets Femke, Bartholomeus Panis. (30/5/2016). CRYOPRESERVATION OF CASSAVA (MANIHOT ESCULENTA) BY DROPLET VITRIFICATION: INFLUENCE OF PLANT AGE, MERISTEM POSITION AND USED PROTOCOL.en_US
dc.identifier.urihttp://hdl.handle.net/20.500.11766/8184
dc.description.abstractCassava is an important root crop cultivated in the tropics and source of starch and animal feed. Pests, diseases and the occurrence of mutations pose a major threat to its genetic resources. Through cryopreservation, we can protect the wide variety that exists in this species. Cryopreservation, using 2 different droplet vitrification methods was investigated and 3 different accessions of Manihot esculenta ( Cassava) were compared. Apical and axillar meristems were excised from 4, 8 and 16 weeks old in vitro grown plants. Overall viability and shoot growth rates where high ( average of 78.3% and 73.56%). Plant regeneration percentages, however, varied considerably for the 3 accessions ( between 1.26% and 30.9%; average of 11.7%). Significantly higher viability, shoot growth and plant regeneration percentages were observed for apical shoot tips compared to axillar shoot tips, except when the fast protocol was used. In general the apical shoot tips provided viability, shoot growth and plant regeneration percentages with an average of respectively 79.5%, 76.7% and 16.6%. For axillar shoot tips this was respectively an average of 76.9%, 70.4% and 6.8%. The fast protocol increased the viability, shoot growth and plant regeneration of axillar shoot tips significantly. When looking at the age of the starting material, a significantly lower viability and shoot growth was observed for 16 weeks old material compared to 4 and 8 weeks old material, but there was no difference in plant regeneration.en_US
dc.formatPDFen_US
dc.languageenen_US
dc.subjectmanihot esculentaen_US
dc.subjectCassavaen_US
dc.titleCRYOPRESERVATION OF CASSAVA (MANIHOT ESCULENTA) BY DROPLET VITRIFICATION: INFLUENCE OF PLANT AGE, MERISTEM POSITION AND USED PROTOCOLen_US
dc.typeThesisen_US
cg.creator.ID0000-0001-6717-947Xen_US
cg.creator.ID-typeORCIDen_US
cg.subject.agrovoccryopreservationen_US
cg.contributor.centerCatholic university Leuven - KULeuvenen_US
cg.contributor.centerBioversity International - Bioversityen_US
cg.contributor.crpCRP on Roots, Tubers and Bananas - RTBen_US
cg.contributor.funderCGIAR System Office - CGIAR - Sysen_US
cg.contributor.project-lead-instituteBioversity International - Bioversityen_US
cg.date.embargo-end-date2022-12-30en_US
cg.coverage.regionWestern Europeen_US
cg.coverage.regionSouth Americaen_US
cg.coverage.countryBEen_US
cg.coverage.countryCOen_US
cg.contactbart.panis@biw.kuleuven.been_US
dc.identifier.statusLimited accessen_US


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record


DSpace software copyright © 2002-2015  DuraSpace
Disclaimer:
MELSpace content providers and partners accept no liability to any consequence resulting from use of the content or data made available in this repository. Users of this content assume full responsibility for compliance with all relevant national or international regulations and legislation.
Theme by 
@mire NV