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dc.contributorPothana, Arunimaen_US
dc.contributorPrasad, Kalyanien_US
dc.contributorShah, Dilipen_US
dc.contributorKaur, Jagdeepen_US
dc.contributorBhatnagar, Deepaken_US
dc.contributorChen, Zhi-Yuanen_US
dc.contributorRaruang, Yenjiten_US
dc.contributorCary, Jeffreyen_US
dc.contributorRajasekaran, Kanniahen_US
dc.contributorSudini, Hari kishanen_US
dc.contributorBhatnagar - Mathur, Poojaen_US
dc.creatorSharma, Kiranen_US
dc.identifier.citationKiran Sharma, Arunima Pothana, Kalyani Prasad, Dilip Shah, Jagdeep Kaur, Deepak Bhatnagar, Zhi-Yuan Chen, Yenjit Raruang, Jeffrey Cary, Kanniah Rajasekaran, Hari kishan Sudini, Pooja Bhatnagar - Mathur. (1/5/2018). Peanuts that keep aflatoxin at bay: a threshold that matters. Plant Biotechnology Journal, 16(5), pp. 1024-1033.en_US
dc.description.abstractAflatoxin contamination in peanuts poses major challenges for vulnerable populations of subSaharan Africa and South Asia. Developing peanut varieties to combat preharvest Aspergillus flavus infection and resulting aflatoxin contamination has thus far remained a major challenge, confounded by highly complex peanut–Aspergilli pathosystem. Our study reports achieving a high level of resistance in peanut by overexpressing (OE) antifungal plant defensins MsDef1 and MtDef4.2, and through host-induced gene silencing (HIGS) of aflM and aflP genes from the aflatoxin biosynthetic pathway. While the former improves genetic resistance to A. flavus infection, the latter inhibits aflatoxin production in the event of infection providing durable resistance against different Aspergillus flavus morphotypes and negligible aflatoxin content in several peanut events/lines well. A strong positive correlation was observed between aflatoxin accumulation and decline in transcription of the aflatoxin biosynthetic pathway genes in both OE-Def and HIGS lines. Transcriptomic signatures in the resistant lines revealed key mechanisms such as regulation of aflatoxin synthesis, its packaging and export control, besides the role of reactive oxygen species-scavenging enzymes that render enhanced protection in the OE and HIGS lines. This is the first study to demonstrate highly effective biotechnological strategies for successfully generating peanuts that are near-immune to aflatoxin contamination, offering a panacea for serious food safety, health and trade issues in the semi-arid regions.en_US
dc.publisherWiley Open Accessen_US
dc.sourcePlant Biotechnology Journal;16,(2017) Pagination 1024,1033en_US
dc.subjectaspergillus flavusen_US
dc.subjecthost-induced gene silencingen_US
dc.titlePeanuts that keep aflatoxin at bay: a threshold that mattersen_US
dc.typeJournal Articleen_US
cg.subject.agrovocfood safetyen_US
cg.contributor.centerInternational Crops Research Institute for the Semi-Arid Tropics - ICRISATen_US
cg.contributor.centerDonald Danforth Plant Science Centeren_US
cg.contributor.centerUnited States Department of Agriculture, Agricultural Research Service, The Southern Regional Research Center - USDA - ARS - SRRCen_US
cg.contributor.centerLouisiana State University - LSU Louisianaen_US
cg.contributor.crpCRP on Grain Legumes and Dryland Cereals - GLDCen_US
cg.contributor.funderCGIAR System Office - CGIAR - Sysen_US
cg.contributor.project-lead-instituteCRP on Dryland Systems - DSen_US
dc.identifier.statusOpen accessen_US

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