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dc.contributorThami Alami, Imaneen_US
dc.contributorDouira, Allalen_US
dc.contributorUdupa, Sripada M.en_US
dc.creatorBenjelloun, Imaneen_US
dc.identifier.citationImane Benjelloun, Imane Thami Alami, Allal Douira, Sripada M. Udupa. (18/9/2019). Phenotypic and Genotypic Diversity Among Symbiotic and Non-symbiotic Bacteria Present in Chickpea Nodules in Morocco. Frontiers in Microbiology, 10: 1885, pp. 1-19.en_US
dc.description.abstractEnvironmental pollution problems and increased demand for green technologies in production are forcing farmers to introduce agricultural practices with a lower impact on the environment. Chickpea (Cicer arietinum) in arid and semi-arid environments is frequently affected by harsh environmental stresses such as heat, drought and salinity, which limit its growth and productivity and affect biological nitrogen fixation ability of rhizobia. Climate change had further aggravated these stresses. Inoculation with appropriate stress tolerant rhizobia is necessary for an environmentally friendly and sustainable agricultural production. In this study, endophytic bacteria isolated from chickpea nodules from different soil types and regions in Morocco, were evaluated for their phenotypic and genotypic diversity in order to select the most tolerant ones for further inoculation of this crop. Phenotypic characterization of 135 endophytic bacteria from chickpea nodules showed a wide variability for tolerance to heavy metals and antibiotics, variable response to extreme temperatures, salinity, pH and water stress. 56% of isolates were able to nodulate chickpea. Numerical analysis of rep-PCR results showed that nodulating strains fell into 22 genotypes. Sequencing of 16S rRNA gene of endophytic bacteria from chickpea nodules revealed that 55% of isolated bacteria belong to Mesorhizobium genus. Based on MLSA of core genes (recA, atpD, glnII and dnaK), tasted strains were distributed into six clades and were closely related to Mesorhizobium ciceri, Mesorhizobium opportunistum, Mesorhizobium qingshengii, and Mesorhizobium plurifarium. Most of nodulating strains were belonging to a group genetically distinct from reference Mesorhizobium species. Three isolates belong to genus Burkholderia of the class β- proteobacteria, and 55 other strains belong to the class γ- proteobacteria. Some of the stress tolerant isolates have great potential for further inoculation of chickpea in the arid and semiarid environments to enhance biological nitrogen fixation and productivity in the context of climate change adaptation and mitigation.en_US
dc.publisherFrontiers Mediaen_US
dc.sourceFrontiers in Microbiology;10:1885,(2019) Pagination 1-19en_US
dc.subjectendophytic bacteriaen_US
dc.subjectgamma proteobacteriaen_US
dc.subjectbeta proteobacteriaen_US
dc.titlePhenotypic and Genotypic Diversity Among Symbiotic and Non-symbiotic Bacteria Present in Chickpea Nodules in Moroccoen_US
dc.typeJournal Articleen_US
cg.creator.idUdupa, Sripada M.: 0000-0003-4225-7843en_US
cg.subject.agrovoccicer arietinumen_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.centerNational Institute of Agronomic Research Morocco - INRA Moroccoen_US
cg.contributor.centerIbn Tofail University, Faculty of Sciences - UIT - FSen_US
cg.contributor.centerIbn Tofail University - UIT Moroccoen_US
cg.contributor.crpCGIAR Research Program on Dryland Systems - DSen_US
cg.contributor.funderGovernment of Morocco - Moroccoen_US
cg.contributor.projectMoroccan Collaborative Grants Program (MCGP)en_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.coverage.regionNorthern Africaen_US
cg.isijournalISI Journalen_US
dc.identifier.statusOpen accessen_US
cg.journalFrontiers in Microbiologyen_US

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