| dc.contributor | Gaboun, F. | en_US |
| dc.contributor | Abdelwahd, Rabha | en_US |
| dc.contributor | Udupa, Sripada M. | en_US |
| dc.contributor | Douira, Allal | en_US |
| dc.contributor | Iraqi, Driss | en_US |
| dc.creator | Senhaji, Chaimae | en_US |
| dc.date | 2019-10-31 | en_US |
| dc.date.accessioned | 2019-11-11T21:24:31Z | |
| dc.date.available | 2019-11-11T21:24:31Z | |
| dc.identifier | https://mel.cgiar.org/reporting/download/hash/efd377cf317d24f8bf772a53317525a7 | en_US |
| dc.identifier.citation | Chaimae Senhaji, F. Gaboun, Rabha Abdelwahd, Sripada M. Udupa, Allal Douira, Driss Iraqi. (31/10/2019). Development of an efficient regeneration system for bombarded calli from immature embryos of Moroccan durum wheat varieties. Agronomy Research, 17. | en_US |
| dc.identifier.uri | https://hdl.handle.net/20.500.11766/10388 | |
| dc.description.abstract | One of the biggest obstacles limiting genetic transformation of durum wheat is the lack of an efficient regeneration system for bombarded tissues. Our study aims to optimize culture conditions for regenerating bombarded calli from immature embryos of four durum wheat varieties ‘Amria’, ‘Chaoui’, ‘Isly’ and ‘Marouane’, through comparing the effects of phytohormones (IAA, zeatin and their interaction) and nitrogen amount and sources on callus induction and plant regeneration. Both tested induction media induced approximately the same rate of induced calli for all the tested varieties. However, the interaction of the induction and the regeneration media showed a highly significant effect on plantlet regeneration for all tested varieties. After bombardment, IM1/RM2 combination proved to be the favourable medium with up to 200% and 120% plantlets regenerated for ‘Chaoui’ and ‘Isly’ varieties respectively. Encouraging results obtained in this study will help to promote the research in genetic transformation and its improvement. | en_US |
| dc.format | PDF | en_US |
| dc.language | en | en_US |
| dc.publisher | Estonian University of Life Sciences | en_US |
| dc.rights | CC-BY-NC-ND-4.0 | en_US |
| dc.source | Agronomy Research;17,(2019) | en_US |
| dc.subject | plantlets regeneration | en_US |
| dc.subject | bombarded calli | en_US |
| dc.subject | callus induction | en_US |
| dc.subject | immature embryos | en_US |
| dc.title | Development of an efficient regeneration system for bombarded calli from immature embryos of Moroccan durum wheat varieties | en_US |
| dc.type | Journal Article | en_US |
| cg.creator.id | Udupa, Sripada M.: 0000-0003-4225-7843 | en_US |
| cg.subject.agrovoc | durum (triticum durum) | en_US |
| cg.subject.agrovoc | Durum Wheat | en_US |
| cg.contributor.center | International Center for Agricultural Research in the Dry Areas - ICARDA | en_US |
| cg.contributor.center | National Institute of Agronomic Research Morocco - INRA Morocco | en_US |
| cg.contributor.center | Ibn Tofail University, Faculty of Sciences - UIT - FS | en_US |
| cg.contributor.crp | CGIAR Research Program on Wheat - WHEAT | en_US |
| cg.contributor.funder | International Maize and Wheat Improvement Center - CIMMYT | en_US |
| cg.contributor.project | CRP WHEAT Phase II | en_US |
| cg.contributor.project-lead-institute | International Center for Agricultural Research in the Dry Areas - ICARDA | en_US |
| cg.coverage.region | Northern Africa | en_US |
| cg.coverage.country | MA | en_US |
| cg.contact | chaimae.310@gmail.com | en_US |
| cg.identifier.doi | https://dx.doi.org/10.15159/ar.19.191 | en_US |
| dc.identifier.status | Open access | en_US |
| cg.issn | 1406-894X | en_US |
| cg.journal | Agronomy Research | en_US |
| cg.volume | 17 | en_US |
