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dc.contributorGaboun, F.en_US
dc.contributorAbdelwahd, Rabhaen_US
dc.contributorUdupa, Sripada M.en_US
dc.contributorDouira, Allalen_US
dc.contributorIraqi, Drissen_US
dc.creatorSenhaji, Chaimaeen_US
dc.identifier.citationChaimae Senhaji, F. Gaboun, Rabha Abdelwahd, Sripada M. Udupa, Allal Douira, Driss Iraqi. (31/10/2019). Development of an efficient regeneration system for bombarded calli from immature embryos of Moroccan durum wheat varieties. Agronomy Research, 17.en_US
dc.description.abstractOne of the biggest obstacles limiting genetic transformation of durum wheat is the lack of an efficient regeneration system for bombarded tissues. Our study aims to optimize culture conditions for regenerating bombarded calli from immature embryos of four durum wheat varieties ‘Amria’, ‘Chaoui’, ‘Isly’ and ‘Marouane’, through comparing the effects of phytohormones (IAA, zeatin and their interaction) and nitrogen amount and sources on callus induction and plant regeneration. Both tested induction media induced approximately the same rate of induced calli for all the tested varieties. However, the interaction of the induction and the regeneration media showed a highly significant effect on plantlet regeneration for all tested varieties. After bombardment, IM1/RM2 combination proved to be the favourable medium with up to 200% and 120% plantlets regenerated for ‘Chaoui’ and ‘Isly’ varieties respectively. Encouraging results obtained in this study will help to promote the research in genetic transformation and its improvement.en_US
dc.publisherEstonian University of Life Sciencesen_US
dc.sourceAgronomy Research;17,(2019)en_US
dc.subjectplantlets regenerationen_US
dc.subjectbombarded callien_US
dc.subjectcallus inductionen_US
dc.subjectimmature embryosen_US
dc.titleDevelopment of an efficient regeneration system for bombarded calli from immature embryos of Moroccan durum wheat varietiesen_US
dc.typeJournal Articleen_US
cg.creator.idUdupa, Sripada M.: 0000-0003-4225-7843en_US
cg.subject.agrovocdurum (triticum durum)en_US
cg.subject.agrovocDurum Wheaten_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.centerNational Institute of Agronomic Research Morocco - INRA Moroccoen_US
cg.contributor.centerIbn Tofail University, Faculty of Sciences - UIT - FSen_US
cg.contributor.crpCGIAR Research Program on Wheat - WHEATen_US
cg.contributor.funderInternational Maize and Wheat Improvement Center - CIMMYTen_US
cg.contributor.projectCRP WHEAT Phase IIen_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.coverage.regionNorthern Africaen_US
dc.identifier.statusOpen accessen_US
cg.journalAgronomy Researchen_US

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