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dc.contributorVarshney, Rajeeven_US
dc.contributorSudini, Hari kishanen_US
dc.contributorPandey, Manish Ken_US
dc.creatorPandey, Arunen_US
dc.date2019-06-05en_US
dc.date.accessioned2020-02-11T11:28:42Z
dc.date.available2020-02-11T11:28:42Z
dc.identifierhttps://mel.cgiar.org/reporting/download/hash/940d01c008667c910e7a47eea81ac0e3en_US
dc.identifier.citationArun Pandey, Rajeev Varshney, Hari kishan Sudini, Manish K Pandey. (5/6/2019). An Improved Enzyme-Linked Immunosorbent Assay (ELISA) Based Protocol Using Seeds for Detection of Five Major Peanut Allergens Ara h 1, Ara h 2, Ara h 3, Ara h 6, and Ara h 8. Frontiers in Nutrition, 68(6), pp. 1-11.en_US
dc.identifier.urihttps://hdl.handle.net/20.500.11766/10713
dc.description.abstractPeanut allergy is an important health concern among many individuals. As there is no effective treatment to peanut allergy, continuous monitoring of peanut-based products, and their sources is essential. Precise detection of peanut allergens is key for identification and development of improved peanut varieties with minimum or no allergens in addition to estimating the levels in peanut-based products available in food chain. The antibody based ELISA protocol along with sample preparation was standardized for Ara h 1, Ara h 2, Ara h 3, Ara h 6, and Ara h 8 to estimate their quantities in peanut seeds. Three different dilutions were optimized to precisely quantify target allergen proteins in peanut seeds such as Ara h 1 (1/1,000, 1/2,000, and 1/4,000), Ara h 2 and Ara h 3 (1/5,000, 1/10,000, and 1/20,000), Ara h 6 (1/40,000, 1/80,000, and 1/1,60,000), and Ara h 8 (1/10, 1/20, and 1/40). These dilutions were finalized for each allergen based on the accuracy of detection by achieving <20% coefficient of variation in three technical replicates. This protocol captured wide variation of allergen proteins in selected peanut genotypes for Ara h 1 (77–46,106 μg/g), Ara h 2 (265–5,426 μg/g), Ara h 3 (382–12,676 μg/g), Ara h 6 (949–43,375 μg/g), and Ara h 8 (0.385–6 μg/g). The assay is sensitive and reliable in precise detection of five major peanut allergens in seeds. Deployment of such protocol allows screening of large scale germplasm and breeding lines while developing peanut varieties with minimum allergenicity to ensure food safety.en_US
dc.formatTXTen_US
dc.languageenen_US
dc.publisherFrontiers Mediaen_US
dc.rightsCC-BY-NC-4.0en_US
dc.sourceFrontiers in Nutrition;68,(2019) Pagination 1,11en_US
dc.subjectgroundnuten_US
dc.subjectprotocolen_US
dc.subjectpeanut allergensen_US
dc.subjectara h 1en_US
dc.subjectara h 2en_US
dc.subjectara h 3en_US
dc.subjectara h 6en_US
dc.subjectara h 8en_US
dc.subjectGroundnuten_US
dc.titleAn Improved Enzyme-Linked Immunosorbent Assay (ELISA) Based Protocol Using Seeds for Detection of Five Major Peanut Allergens Ara h 1, Ara h 2, Ara h 3, Ara h 6, and Ara h 8en_US
dc.typeJournal Articleen_US
cg.creator.idPandey, Manish K: 0000-0002-4101-6530en_US
cg.creator.ID-typeORCIDen_US
cg.subject.agrovocfood safetyen_US
cg.subject.agrovocelisaen_US
cg.subject.agrovocfood allergiesen_US
cg.contributor.centerInternational Crops Research Institute for the Semi-Arid Tropics - ICRISATen_US
cg.contributor.crpCRP on Grain Legumes and Dryland Cereals - GLDCen_US
cg.contributor.funderMars Wrigley Confectioneryen_US
cg.contributor.projectIdentification of Markers and Genomic Regions Associated with Aflotoxin Resistance in Peanuten_US
cg.contributor.project-lead-instituteInternational Crops Research Institute for the Semi-Arid Tropics - ICRISATen_US
cg.coverage.regionSouthern Asiaen_US
cg.coverage.countryINen_US
cg.contactM.Pandey@cgiar.orgen_US
cg.identifier.doihttps://dx.doi.org/10.3389/fnut.2019.00068en_US
dc.identifier.statusOpen accessen_US
mel.impact-factor0en_US


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