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dc.contributorGupta, Priyankaen_US
dc.contributorSingh, Murarien_US
dc.contributorAgrawal, Shiv Kumaren_US
dc.creatorBarpete, Surendraen_US
dc.date2020-07-15en_US
dc.date.accessioned2020-10-15T21:27:42Z
dc.date.available2020-10-15T21:27:42Z
dc.identifierhttps://mel.cgiar.org/dspace/limiteden_US
dc.identifier.citationSurendra Barpete, Priyanka Gupta, Murari Singh, Shiv Kumar Agrawal. (15/7/2020). Culture selected somaclonal variants showing low-ODAP and high protein content in nineteen grass pea (Lathyrus sativus L. ) genotypes. Tissue and Organ Culture (PCTOC), 142, pp. 625-634.en_US
dc.identifier.urihttps://hdl.handle.net/20.500.11766/11902
dc.description.abstractTo develop low-ODAP grass pea genotypes with high protein content, in vitro tissue culture techniques were used for inducing somaclonal variation through internode explants that was cultured on B5 medium containing 2 mg L−l α-naphthaleneacetic acid and 1 mg L− l 6-benzylaminopurine of 19 grass pea genotypes. The selected somaclones ( R3 and R4 generations) were evaluated for variation in ODAP, protein, and quantitative agronomic traits. The results showed statistically significant (P < 0.001) genotypic differences for ODAP %, Protein %, 100-seed weight (HSW) and pod width (PODW) in the R4 somaclones under field conditions. The somaclones showed broad-sense heritability estimate of 0.14 ± 0.064 for ODAP%, 0.87 ± 0.015 for protein, 0.78 ± 0.025 for HSW and 0.13 ± 0.062 for PODW. Among the 270 somaclones, Sel462-5 was significantly superior to the parent and other somaclones in terms of ODAP content (0.13%), protein content (28.47%) and pod width (1.43 cm). The correlation coefficients between parents and somaclones for pod width with 100-seed weight, ODAP-clones and protein-clones were positive and significant. The superiority of somaclones for ODAP, protein, 100-seed weight and pod width in R4 generation opened the possibility for grass pea improvement through somaclonal variation.en_US
dc.formatPDFen_US
dc.languageenen_US
dc.publisherSpringer (part of Springer Nature)en_US
dc.rightsAll right reserveden_US
dc.sourceTissue and Organ Culture (PCTOC);142,(2020) Pagination 625,634en_US
dc.subjectproteinen_US
dc.subjectlathyrus sativus l.en_US
dc.titleCulture selected somaclonal variants showing low-ODAP and high protein content in nineteen grass pea (Lathyrus sativus L.) genotypesen_US
dc.typeJournal Articleen_US
cg.creator.idBarpete, Surendra: 0000-0002-5547-8488en_US
cg.creator.idSingh, Murari: 0000-0001-5450-0949en_US
cg.creator.idAgrawal, Shiv Kumar: 0000-0001-8407-3562en_US
cg.creator.ID-typeORCIDen_US
cg.creator.ID-typeORCIDen_US
cg.creator.ID-typeORCIDen_US
cg.subject.agrovocgrass peaen_US
cg.subject.agrovocsomaclonal variationen_US
cg.subject.agrovoctissue cultureen_US
cg.subject.agrovocodapen_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.centerConcordia Universityen_US
cg.contributor.funderIndian Council of Agricultural Research - ICARen_US
cg.contributor.projectIndia Collaborative Program: Restricted funding for breeding for resistance to abiotic stresses in pulses & for 2017/2018 - 2017/2020 - 2020/2021en_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.date.embargo-end-dateTimelessen_US
cg.coverage.regionGlobalen_US
cg.contactsurendrabarpete@gmail.comen_US
cg.identifier.doihttps://dx.doi.org/10.1007/s11240-020-01889-0en_US
dc.identifier.statusTimeless limited accessen_US
mel.project.openhttp://geoagro.icarda.org/india/en_US
mel.impact-factor2.196en_US


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