Molecular detection of Toxoplasma gondii and Sarcocystis spp. co-infection in Tunisian Merguez, a traditional processed sausage beef meat
Impact factor: 5.548 (Year: 2021)
MetadataShow full item record
Timeless limited access
Safa Amairia, Mariem Rouatbi, Mohamed Ridha Frikha, Jacinto Gomes, Mourad Rekik, Mohamed Aziz Darghouth, Mohamed Gharbi. (1/3/2021). Molecular detection of Toxoplasma gondii and Sarcocystis spp. co-infection in Tunisian Merguez, a traditional processed sausage beef meat. Food Control, 121.
With the increase of raw food consumption, there is a growing interest in the risk posed by zoonotic infections to human health. This study was performed to identify in popular processed beef meat-based sausages (Merguez) the prevalence of Toxoplasma gondii and the zoonotic species of Sarcocystis. A total of 99 samples, collected from four Tunisian regions, were used for molecular analyses. For each sample, DNA was extracted from 4 equal pieces and PCR reactions were performed. Phylogenetic analyses were performed to identify Sarcocystis species. Prevalences of T. gondii and Sarcocystis spp. DNA in Merguez samples were 47.5% (95% CI: 0.38–0.57) and 52.5% (95% CI: 0.43–0.62), respectively. Important differences between regions were found. The DNA of both parasites was detected in 14.1% of the same samples. For Sarcocystis spp. infection, the percentage of samples having one positive piece was significantly higher than those having two, three or four positive pieces. Whereas, for T. gondii, the majority of tested samples had two positive pieces (16.2%) (p = 0.002). Sequencing showed the presence of Sarcocystis tenella and Sarcocystis cruzi species. Our findings suggest a high prevalence of T. gondii and Sarcocystis spp. in Merguez sausages with a potential high human health risk if the latter are undercooked.
- Agricultural Research Knowledge 
Showing items related by title, author, creator and subject.
Title: The database of the PREDICTS (Projecting Responses of Ecological Diversity In Changing Terrestrial Systems) project Author(s)Hudson, Lawrence N.; Newbold, Tim; Contu, Sara; Hill, Samantha; Lysenko, Igor; De Palma, Adriana; Phillips, Helen; Alhusseini, Tamera; Bedford, Felicity; Bennett, Dominic; Booth, Hollie; Burton, Victoria; Chng, Charlotte; Choimes, Argyrios; Correia, David L. P.; Day, Julie; Echeverria-Londono, Susy; Emerson, Susan; Gao, Di; Garon, Morgan; Harrison, Michelle; Ingram, Daniel; Jung, Martin; Kemp, Victoria; Kirkpatrick, Lucinda; Martin, Callum D.; Pan, Yuan; Pask-Hale, Gwilym; Pynegar, Edwin; Robinson, Alexandra; Sanchez-Ortiz, Katia; Senior, Rebecca; Simmons, Benno; White, Hannah; Zhang, Hanbin; Aben, Job; Abrahamczyk, Stefan; Adum, Gilbert; Aguilar-Barquero, Virginia; Aizen, Marcelo; Albertos, Belén; Alcala, E.; del Mar Alguacil, Maria; Alignier, Audrey; Ancrenaz, Marc; Andersen, Alan; Arbeláez-Cortés, Enrique; Armbrecht, Inge; Arroyo-Rodríguez, Víctor; Aumann, Tom; Axmacher, Jan; Azhar, Badrul; Azpiroz, Adrián; Baeten, Lander; Bakayoko, Adama; Báldi, András; Banks, John; Baral, Sharad; Barlow, Jos; Barratt, Barbara; Barrico, Lurdes; Bartolommei, Paola; Barton, Diane; Basset, Yves; Batáry, Peter; Bates, Adam; Louhaichi, Mounir; Katovai, Eric; Kellner, Klaus; Kessler, Michael; R Kirby, Kathryn; E Knight, Mairi; Knop, Eva; Koivula, Matti; Kolb, Annette; Kone, Mouhamadou; Korosi, adam; Krauss, Jochen; J. Fonte, Steven; Fournier, Anne; E Fowler, Robert; Franzen, Markus; H Fraser, Lauchlan; M. Fredriksson, Gabriella; B Freire Jr, Geraldo; L. M. Frizzo, Tiago; Furlani, Dario; Gaigher, Rene; U Ganzhorn, Jorg; P. Garcia, Karla; C. Garcia R, Juan; G. Garden, Jenni; Garilleti, Ricardo; Ge, Bao-Ming; Gendreau-Berthiaume, Benoit; J. Gerard, Philippa J. Gerard; Gheler-Costa, Carla; Gilbert, Benjamin; Giordani, Paolo; Giordano, Simonetta; Golodets, Carly; G. L. Gomes, Laurens; K. Gould, Rachelle; Goulson, Dave; Granjon, Laurent; Grass, Ingo; L. Gray, Claudia; Grogan, James; Guardiola, Moises; R Gunawardene, Nihara; G Gutierrez, Alvaro; L Gutierrez Lamus, Doris; H Haarmeyer, Daniela; E Hanley, Mick; R Hashim, Nor; N. Hassan, Shombe; G. Hatfield, Richard; E. Hawes, Joseph; W Hayward, MattDate: 2017-12-31Type: Journal ArticleStatus: Open accessThe PREDICTS project—Projecting Responses of Ecological Diversity In Changing Terrestrial Systems (www.predicts.org.uk)—has collated from published studies a large, reasonably representative database of comparable samples ...
Title: Peer-Reviewed Literature on Grain Legume Species in the WoS (1980–2018): A Comparative Analysis of Soybean and Pulses Author(s)Magrini, Marie-Benoit; Cabanac, Guillaume; Lascialfari, Matteo; Plumecocq, Gaël; Amiot, Marie Josephe; Anton, Marc; Arvisenet, Gaëlle; Baranger, Alain; Bedoussac, Laurent; Chardigny, Jean-Michel; Duc, Gérard; Jeuffroy, Marie-Hélène; Journet, Etienne-Pascal; Juin, Hervé; Larre, Colette; Leiser, Hugues; Micard, Valérie; Millot, Dominique; Pilet-Nayel, Marie-Laure; Nguyen-Thé, Christophe; Salord, Tristan; Voisin, Anne-Sophie; Walrand, Stephane; Wery, JacquesDate: 2019-12-02Type: Journal ArticleStatus: Open accessGrain-legume crops are important for ensuring the sustainability of agrofood systems. Among them, pulse production is subject to strong lock-in compared to soya, the leading worldwide crop. To unlock the situation and ...
Author(s)Udupa, Sripada M.Date: 2021-06-30Type: ThesisStatus: Timeless limited accessThis study was carried out at the Sidi El Aidi Experimental Station during the 2020/2021 agricultural campaign and at the INRA / ICARDA Biotechnology laboratory, with the main objective of studying the genetic diversity ...