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dc.contributorSharman, Murrayen_US
dc.contributorFilardo, Fionaen_US
dc.contributorMoukahel, Abdulrahmanen_US
dc.contributorNader, Asaaden_US
dc.contributorMghandef, Samiaen_US
dc.contributorVan Leur, Joopen_US
dc.creatorKumari, Safaaen_US
dc.date2019-11-25en_US
dc.date.accessioned2021-02-23T23:22:13Z
dc.date.available2021-02-23T23:22:13Z
dc.identifierhttps://era.daf.qld.gov.au/id/eprint/7163/en_US
dc.identifierhttps://mel.cgiar.org/reporting/download/hash/5d46344f58c801d4fcc381a9ef4a7f09en_US
dc.identifier.citationKumari, S. G. M. Sharman, F. Filardo, A. -R. Moukahel, N. Asaad, S. Mghandef and J. van Leur. 2019. Molecular Diagnosis of Viruses Causing Yellowing and Stunting Symptoms Affecting Pulse Crops in Central West Asia and North Africa Countries. Page 150. In: Abstracts book of Australasian Plant Pathology Society Conference (APPS2019), 25-28 November 2019, Melbourne, Australia.en_US
dc.identifier.urihttps://hdl.handle.net/20.500.11766/12568
dc.description.abstractViruses causing yellowing and stunting are the most important virus diseases affecting pulses in many regions of the world, and were considered for many years to be caused mainly by infection with beet western yellows virus (BWYV, genus Polerovirus, family Luteoviridae). Knowing the exact identity of a virus affecting pulse crops is essential for breeding for resistance and crop management purposes. More than 5000 pulse samples (faba bean, lentil, and chickpea) with symptoms typical of virus infection including stunting, yellowing, necrosis and reddening were collected during the last two decades from different countries in Central West Asia and North Africa (CWANA). All samples were tested serologically by tissue-blot immunoassay (TBIA) technique using specific luteoviridae monoclonal antibodies. Selected samples were further tested by reverse transcription polymerase chain reaction (RT-PCR) using different luteoviridae primer pairs (generic and specific) followed by amplicon sequencing. RT-PCR results revealed clearly that there was a greater variation in polerovirus species detected than was indicated by TBIA alone. Molecular diagnosis has clearly shown that there are a number of Polerovirus species, in addition to BWYV (detected in Algeria, Ethiopia, Lebanon, Morocco, Sudan, Tunisia and Uzbekistan), each of which can produce yellowing and stunting symptoms in pulses in CWANA. These viruses are cucurbit aphid-borne yellows virus (detected in Algeria, Lebanon, Syria, Sudan and Uzbekistan), chickpea chlorotic stunt virus (detected in Algeria, Ethiopia, Lebanon, Morocco, Syria, and Tunisia), pepper vein yellows virus (detected in Morocco and Sudan), pepo aphid-borne yellows virus (detected in Sudan), and cotton leafroll dwarf virus (detected in Sudan and Uzbekistan). This study clearly showed that molecular characterization is an essential tool for accurate identification of plant viruses, which is the first step towards better crop management.en_US
dc.formatPDFen_US
dc.languageenen_US
dc.publisherAustralasian Plant Pathology Societyen_US
dc.rightsCC-BY-4.0en_US
dc.sourceNovember 25-28, 2019en_US
dc.subjectluteovirusesen_US
dc.subjectFaba beanen_US
dc.subjectLentilen_US
dc.subjectChickpeaen_US
dc.titleMolecular Diagnosis of Viruses Causing Yellowing and Stunting Symptoms Affecting Pulse Crops in Central West Asia and North Africa Countriesen_US
dc.typeConference Paperen_US
cg.creator.idKumari, Safaa: 0000-0002-4492-6257en_US
cg.creator.ID-typeORCIDen_US
cg.subject.agrovocplant virusesen_US
cg.subject.agrovocdiagnosisen_US
cg.contributor.centerNational Agricultural Research Institute of Tunisia - INRATen_US
cg.contributor.centerGeneral Commission for Scientific Agricultural Research - GCSARen_US
cg.contributor.centerNSW Department of Primary Industries - NSW-DPIen_US
cg.contributor.centerDepartment of Agriculture and Fisheries - dafen_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.crpCGIAR Research Program on Grain Legumes and Dryland Cereals - GLDCen_US
cg.contributor.funderArab Fund for Economic and Social Development - AFESDen_US
cg.contributor.funderNSW Department of Primary Industries - NSW-DPIen_US
cg.contributor.projectFellowships Program and post graduate scholarships for implementing and managing agricultural research in the Arab countriesen_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.coverage.regionWestern Asiaen_US
cg.coverage.countryLBen_US
cg.coverage.admin-unitJabal Lubnanen_US
cg.coverage.geolocationLatitude: 33.797259478560136; Longitude: 35.51791034939233en_US
cg.contactS.KUMARI@CGIAR.ORGen_US
dc.identifier.statusOpen accessen_US
mel.project.openhttps://mel.cgiar.org/projects/fellowshipprogramafesden_US


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