Genetic structuration of tick Tunisian populations and the effect of plant essential oils on symbiotic bacteria diversity

Abstract

Objectives - Study by NGS the diversity of Hyalomma tick's microbiota from different species, regions, stages, sex, hosts and the genus of Haemaphysalis and Rhipiciphalus collected from cattle considering the dominant phylogenic characteristics of Tunisian Hyalomma ticks. - Assess the effects of selected essential oils on the reproductive fitness of Hyalomma ticks and investigate their effects on the endosymbionts using real-time PCR.

Expected final deliverables - New scientific knowledge on the major Hyalomma tick symbionts in Tunisia, their diversity and geographical distribution in relation to the species studied, - More advanced experimental knowledge package on the effect of plan essential oils on tick reproduction and development and their potential of application as tick population control alternatives, - New experimental knowledge package on the effects of plant essential oils on tick symbionts, - Construction of an experimental basic prototype (active ingredient, active doses, mode(s) of application, target tick stages, costs, …) targeting the control of tick populations using plant essential oils, through the translation of the experimental knowledge packages into technological components.