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dc.contributorMoreau, Carolen_US
dc.contributorAmbrose, Mikeen_US
dc.contributorIsaac, Peteren_US
dc.contributorEllis, Noelen_US
dc.contributorDomoney, Claireen_US
dc.creatorRayner, Traceyen_US
dc.identifier.citationTracey Rayner, Carol Moreau, Mike Ambrose, Peter Isaac, Noel Ellis, Claire Domoney. (6/6/2017). Genetic Variation Controlling Wrinkled Seed Phenotypes in Pisum: How Lucky Was Mendel. International Journal of Molecular Sciences, 18 (6).en_US
dc.description.abstractOne of the traits studied by Mendel in pea (Pisum sativum L.) was the wrinkled-seeded phenotype, and the molecular basis for a mutation underlying this phenotype was discovered in the 1990s. Although the starch-branching enzyme gene mutation identified at the genetic locus r is most likely to be that in seeds available to Mendel in the mid-1800s, it has remained an open question as to whether or not additional natural mutations in this gene exist within Pisum germplasm collections. Here, we explore this question and show that all but two wrinkled-seeded variants in one such collection correspond to either the mutant allele described previously for the r locus or a mutation at a second genetic locus, rb, affecting the gene encoding the large subunit of Adenosine diphosphoglucose (ADP-glucose) pyrophosphorylase; the molecular basis for the rb mutation is described here. The genetic basis for the phenotype of one (JI 2110) of the two lines which are neither r nor rb has been studied in crosses with a round-seeded variant (JI 281); for which extensive genetic marker data were expected. In marked contrast to the trait studied by Mendel and the rb phenotype; the data suggest that the wrinkled-seeded phenotype in JI 2110 is maternally determined, controlled by two genetic loci, and the extent to which it is manifested is very sensitive to the environment. Metabolite analysis of the cotyledons of JI 2110 revealed a profile for sucrose and sucrose-derived compounds that was more similar to that of wild-type round-seeded, than that of wrinkled-seeded r, pea lines. However, the metabolite profile of the seed coat (testa) of JI 2110 was distinct from that of other round-seeded genotypes tested which, together with analysis of recombinant inbred progeny lines, suggests an explanation for the seed phenotype.en_US
dc.sourceInternational Journal of Molecular Sciences;18,(2017)en_US
dc.subjectpea germplasmen_US
dc.subjectr and rb mutationsen_US
dc.subjectseed phenotypeen_US
dc.subjectseed coat (testa) metabolitesen_US
dc.subjectwrinkled seedsen_US
dc.subjectField pea (Pisum sativum)en_US
dc.titleGenetic Variation Controlling Wrinkled Seed Phenotypes in Pisum: How Lucky Was Mendel?en_US
dc.typeJournal Articleen_US
cg.subject.agrovocgenetic markersen_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.centerJohn Innes Centre, Norwich Research Park - JIC - NRPen_US
cg.contributor.centerUniversity of Auckland - AUKLANDen_US
cg.contributor.funderBiotechnology and Biological Sciences Research Council - BBSRCen_US
cg.contributor.funderEuropean Union - EU Belgiumen_US
cg.contributor.funderJohn Innes Foundationen_US
cg.contributor.projectCommunication and Documentation Information Services (CODIS)en_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.isijournalISI Journalen_US
dc.identifier.statusOpen accessen_US
cg.journalInternational Journal of Molecular Sciencesen_US

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