| dc.description.abstract | A field visit was conducted in March 2002 to identify viruses infecting the faba bean (Vicia faba) crop in four governorates (Fayoum, Beni Suef, El-Nobareia and Bihera) in Egypt. A total of 71 faba bean samples with symptoms of viral infection (leaf rolling, yellowing and mosaic) were collected. These were tested at the Virology Laboratory of ICARDA, Syria, for seven viruses using the tissue-blot immunoassay procedure (Makkouk & Comeau, 1994). Specific rabbit polyclonal antisera were used to test for Chickpea chlorotic dwarf virus (CpCDV, genus Mastrevirus, family Geminiviridae; provided by H. J. Vetten, BBA, Braunschweig, Germany); for Broad bean stain virus (BBSV, genus Comovirus, family Comoviridae); for Bean yellow mosaic virus (BYMV, genus Potyvirus, family Potyviridae) and for Pea seed borne mosaic virus (PSbMV, genus Potyvirus, family Potyviridae). In addition, three specific monoclonal antibodies were used to detect Faba bean necrotic yellows virus (FBNYV, genus Nanovirus, family Nanoviridae) (Franz et al., 1996); Bean leaf roll virus (BLRV, family Luteoviridae) (4B10; Katul, 1992) and Beet western yellows virus (BWYV, genus Polerovirus, family Luteoviridae) (ATCC PVAS-647; American Type Culture Collection, Manassas, VA, USA). Serological tests showed that BYMV, an aphid-transmitted and seedborne virus that was identified in 89% of samples tested, was the most common virus. In most of the fields surveyed, BYMV symptoms were noted to occur at high levels (80–100% infection). PSbMV was detected in nine samples (one from Fayoum and eight from Beni Suef); FBNYV was detected in 11 samples (all from Beni Suef). CpCDV, a leafhopper-transmitted virus, was, by contrast, detected in only two samples collected from the El-Nobareia governorate showing leaf rolling, yellowing and stunting. BWYV, BLRV and BBSV were not detected in any of the samples tested. In Egypt, FBNYV, BYMV and PSbMV have previously been reported in faba bean (Makkouk et al., 1994), but this is the first record of CpCDV affecting faba bean in Egypt. CpCDV is the only member of the Geminivirus that is reported to naturally infect faba bean. References Katul L, 1992. Characterization by Serology and Molecular Biology of Bean leaf roll virus and Faba bean necrotic yellows virus. PhD Thesis. Gottingen, Germany: University of Gottingen. Makkouk KM, Comeau A, 1994. Evaluation of various methods for the detection of barley yellow dwarf luteovirus by the tissue-blot immunoassay and its use for BYDV detection in cereals inoculated at different growth stages. European Journal of Plant Pathology 100, 71–80. Makkouk KM, Rizkallah L, Madkour M, El-Sherbeiny M, Kumari SG, Amriti AW, Solh MB, 1994. Survey of faba bean (Vicia faba L.) for viruses in Egypt. Phytopathologia Mediterranea 33, 207–11. | en_US |
