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dc.contributorBos, L.en_US
dc.contributorAzzam, O. I.en_US
dc.contributorKatul, L.en_US
dc.contributorRizkallah, A.en_US
dc.creatorMakkouk, K. M.en_US
dc.date1987-05-31en_US
dc.date.accessioned2021-06-01T14:41:21Z
dc.date.available2021-06-01T14:41:21Z
dc.identifierhttps://mel.cgiar.org/dspace/limiteden_US
dc.identifier.citationK. M. Makkouk, L. Bos, O. I. Azzam, L. Katul, A. Rizkallah. (31/5/1987). Broad bean stain virus: Identification, detectability with ELISA in faba bean leaves and seeds, occurrence in West Asia and North Africa, and possible wild hosts. European Journal of Plant Pathology, 93, pp. 97-106.en_US
dc.identifier.urihttps://hdl.handle.net/20.500.11766/13155
dc.description.abstractDuring a survey of faba bean viruses in West Asia and North Africa a virus was identified as broad bean stain virus (BBSV) based on host reactions, electron microscopy, physical properties and serology. An antiserum to a Syrian isolate was prepared. With this antiserum the direct double antibody sandwich ELISA (DAS-ELISA) and dot-ELISA were very sensitive in detecting BBSV in leaf extracts, ground whole seeds and germinated embryos. Sensitivity was not reduced when the two-day procedure was replaced by a one-day procedure. Using ELISA the virus was detected in 73 out of 589 faba bean samples with virus-like symptoms collected from Egypt (4 out of 70 samples tested), Lebanon (6/44), Morocco (0/7), Sudan (19/254), Syria (36/145) and Tunisia (8/69). This is the first report of BBSV infection of faba bean in Lebanon, Sudan, Syria and Tunisia. Fourteen wild legume species indigenous to Syria were susceptible to BBSV infection, with only two producing obvious symptoms. The virus was found to be seed transmitted in Vicia palaestina.en_US
dc.languageenen_US
dc.publisherSpringer (part of Springer Nature)en_US
dc.rightsCopyrighted; all rights reserveden_US
dc.sourceEuropean Journal of Plant Pathology;93,(1987) Pagination 97,106en_US
dc.subjectfaba beanen_US
dc.subjectbroad beanen_US
dc.subjectdouble antibody sandwichen_US
dc.subjectbean sampleen_US
dc.subjectFaba beanen_US
dc.titleBroad bean stain virus: Identification, detectability with ELISA in faba bean leaves and seeds, occurrence in West Asia and North Africa, and possible wild hostsen_US
dc.typeJournal Articleen_US
cg.subject.agrovocsudanen_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.centerDLO Research Institute for Plant Protectionen_US
cg.contributor.centerAmerican University of Beirut - AUBen_US
cg.contributor.funderInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.projectCommunication and Documentation Information Services (CODIS)en_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.date.embargo-end-dateTimelessen_US
cg.coverage.regionNorthern Africaen_US
cg.coverage.regionWestern Asiaen_US
cg.coverage.countryEGen_US
cg.coverage.countryLBen_US
cg.coverage.countryMAen_US
cg.coverage.countrySYen_US
cg.coverage.countryTNen_US
cg.coverage.countrySDen_US
cg.contactunknownemail@unknown.comen_US
cg.identifier.doihttps://dx.doi.org/10.1007/BF02000560en_US
dc.identifier.statusTimeless limited accessen_US
mel.impact-factor1.582en_US


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