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dc.contributorDondup, Dawaen_US
dc.contributorYuan, Xingmiaoen_US
dc.contributorGu, Fanghongen_US
dc.contributorWang, Deliangen_US
dc.contributorJia, Fengchaoen_US
dc.contributorLin, Zhipingen_US
dc.contributorBaum, Michaelen_US
dc.contributorZhang, Jingen_US
dc.creatorGuo, Ganggangen_US
dc.identifier.citationGanggang Guo, Dawa Dondup, Xingmiao Yuan, Fanghong Gu, Deliang Wang, Fengchao Jia, Zhiping Lin, Michael Baum, Jing Zhang. (12/9/2014). Rare allele of HvLox-1 associated with lipoxygenase activity in barley (Hordeum vulgare L. ). TAG Theoretical and Applied Genetics, 127, pp. 2095-2103.en_US
dc.description.abstractKey message: Identification and allele-specific marker development of a functional SNP of HvLox - 1 which associated with barley lipoxygenase activity. Abstract Improving the stability of the flavor of beer is one of the main objectives in breeding barley for malting, and lipoxygenase-1 (LOX-1) is a key enzyme controlling this trait. In this study, a modified LOX activity assay was used for null LOX-1 mutant screening. Four barley landraces with no detected level of LOX-1 activity were screened from 1,083 barley germplasm accessions from China. The genomic sequence diversity of the HvLox-1 gene of the four null LOX-1 Chinese landraces was compared with that of a further 76 accessions. A total of 104 nucleotide polymorphisms were found, which contained 83 single-nucleotide polymorphisms (SNPs), 7 multiple-nucleotide polymorphisms, and 14 insertions and deletions. Most notably, we found a rare C/G mutation (SNP-61) in the second intron which led to null LOX-1 activity through an altered splicing acceptor site. In addition, an allele-specific polymerase chain reaction marker was developed for the genotyping of SNP-61, which could be used in breeding programs for barley to be used for malting. The objective was to improve beer quality.en_US
dc.publisherSpringer (part of Springer Nature)en_US
dc.sourceTAG Theoretical and Applied Genetics;127,(2014) Pagination 2095,2103en_US
dc.subjectbarley (hordeum vulgare l.)en_US
dc.titleRare allele of HvLox-1 associated with lipoxygenase activity in barley (Hordeum vulgare L.)en_US
dc.typeJournal Articleen_US
cg.creator.idBaum, Michael: 0000-0002-8248-6088en_US
cg.contributor.centerChinese Academy of Agricultural Sciences, Institute of Crop Science - CAAS-ICSen_US
cg.contributor.centerTibet Academy of Agricultural and Animal Husbandry Sciences - TAAASen_US
cg.contributor.centerChina National Research Institute of Food and Fermentation Industriesen_US
cg.contributor.centerBeijing Yanjing Beer Group Co Ltden_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.funderInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.projectCommunication and Documentation Information Services (CODIS)en_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
dc.identifier.statusOpen accessen_US

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