Fusarium vascular wilt in lentil: Inheritance and identification of DNA markers for resistance

Abstract

The inheritance of resistance to lentil (Lens culinaris Medik.) vascular wilt caused by Fusarium oxysporum f.sp, lentis was investigated in a cross between resistant (ILL5588) and susceptible (L692-16-1(s)) lines. F-2:4 progenies and F-6:8, F-6:9 recombinant inbred line (RIL) populations were assessed for their wilt reaction for three seasons in a well-established wilt-sick plot. Resistance to wilt was conditioned by a single dominant gene in the populations studied. The map location of the Fw locus was identified For the first time through linkage to a random amplified polymorphic DNA (RAPD) marker (OPK-15(900)) at 10.8 cM. Two other RAPD markers (OP-B17(800) and OP-D15(500)) identified by bulked segregant analysis were associated in the coupling phase with the resistance trait, and another marker (OP-C04(650)) was associated with repulsion. The DNA markers reported here will provide a starting point in marker-assisted selection for vascular wilt resistance in lentil.