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dc.contributorBaum, Michaelen_US
dc.creatorUdupa, Sripada M.en_US
dc.identifier.citationSripada M. Udupa, Michael Baum. (31/8/2001). High mutation rate and mutational bias at (TAA)n microsatellite loci in chickpea (Cicer arietinum L. ). Molecular Genetics and Genomics, 265, pp. 1097-1103.en_US
dc.description.abstractMicrosatellites, very short tandemly repeated DNA sequences, are being extensively used in evolutionary genetics and molecular breeding of crop plants, because of their high degree of allelic variability, which is presumably caused by a high rate of mutation that changes microsatellite array length. In humans and various animals, mutation rates vary greatly and fall within the range of 10(-3) to 10(-6). In plants, the mutation rate at microsatellite loci seems to be higher than in animals, but no experimental estimates are available yet. Here, we report high spontaneous mutation rates (micro) and mutational bias at fifteen perfect (TAA)n microsatellite loci in inbred populations of chickpea. We show a significantly higher mutation rate, averaged across all loci, in the long-lived variety Ghab 2 (mu = 1.0 x 10(-2); detected in 16,050 allele-generations) compared to the variety Syrian Local (mu = 3.9 x 10(-3); detected in 15,600 allele-generations), which has a short life-span, with the majority of mutants (96.9%) in both varieties differing by < or = 1 repeat unit. Compared to animals, higher mutation rates in chickpea are likely to be due to the presence of long (TAA)n microsatellite repeat arrays and the larger number of DNA replications that meristematic initials of the plants undergo before reaching the reproductive phase. Thus, the long-lived variety undergoes more DNA replications, resulting in an accumulation of more mutations than in the variety with the shorter life-span.en_US
dc.publisherSpringer Natureen_US
dc.rightsCopyrighted; all rights reserveden_US
dc.sourceMolecular Genetics and Genomics;265,(2001) Pagination 1097,1103en_US
dc.titleHigh mutation rate and mutational bias at (TAA)n microsatellite loci in chickpea (Cicer arietinum L.)en_US
dc.typeJournal Articleen_US
cg.creator.idUdupa, Sripada M.: 0000-0003-4225-7843en_US
cg.creator.idBaum, Michael: 0000-0002-8248-6088en_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.funderInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.projectCommunication and Documentation Information Services (CODIS)en_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
dc.identifier.statusTimeless limited accessen_US

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