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dc.contributorUrso, Simonaen_US
dc.contributorBernardo, Letiziaen_US
dc.contributorTondelli, Alessandroen_US
dc.contributorTacconi, Giannien_US
dc.contributorMartino, Valentinaen_US
dc.contributorGrando, Stefaniaen_US
dc.contributorValè, Giampieroen_US
dc.creatorBiselli, Chiaraen_US
dc.identifier.citationChiara Biselli, Simona Urso, Letizia Bernardo, Alessandro Tondelli, Gianni Tacconi, Valentina Martino, Stefania Grando, Giampiero Valè. (1/4/2010). Identification and mapping of the leaf stripe resistance gene Rdg1a in Hordeum spontaneum. TAG Theoretical and Applied Genetics, 120, pp. 1207-1218.en_US
dc.description.abstractLeaf stripe of barley, caused by Pyrenophora graminea, is an important seed-borne disease in organically grown as well as in conventionally grown Nordic and Mediterranean barley districts. Two barley segregating populations represented by 103 recombinant inbred lines (RILs) of the cross L94 (susceptible) × Vada (resistant) and 194 RILs of the cross Arta (susceptible) × Hordeum spontaneum 41-1 (resistant) were analysed with two highly virulent leaf stripe isolates, Dg2 and Dg5, to identify loci for P. graminea resistance. A major gene with its positive allele contributed by Vada and H. spontaneum 41-1 was detected in both populations and for both pathogen isolates on chromosome 2HL explaining 44.1 and 91.8% R 2, respectively for Dg2 and Dg5 in L94 × Vada and 97.8 and 96.1% R 2, respectively for Dg2 and Dg5 in Arta × H. spontaneum 41-1. Common markers in the gene region of the two populations enabled map comparison and highlighted an overlapping for the region of the resistance locus. Since the map position of the resistance locus identified in this report is the same as that for the leaf stripe resistance gene Rdg1a, mapped earlier in Alf and derived from the ‘botanical’ barley line H. laevigatum, we propose that leaf stripe resistance in Vada and H. spontaneum 41-1 is governed by the same gene, namely by Rdg1a, and that Rdg1a resistance could be traced back to H. spontaneum, the progenitor of cultivated barley. PCR-based molecular markers that can be used for marker-assisted selection (MAS) of Rdg1a were identified. An Rdg1a syntenic interval with the rice chromosome arm 4L was identified on the basis of rice orthologs of EST-based barley markers. Analysis of the rice genes annotated into the syntenic interval did not reveal sequences strictly belonging to the major class (nucleotide-binding site plus leucine-rich repeat) of the resistance genes. Nonetheless, four genes coding for domains that are present in the major disease-resistance genes, namely receptor-like protein kinase and ATP/GTP-binding proteins, were identified together with a homolog of the barley powdery mildew resistance gene mlo. Three (out of five) homologs of these genes were mapped in the Rdg1a region in barley and the mlo homolog map position was tightly associated with the LOD score peak in both populations.en_US
dc.publisherSpringer (part of Springer Nature)en_US
dc.sourceTAG Theoretical and Applied Genetics;120,(2009) Pagination 1207-1218en_US
dc.subjectpowdery mildewen_US
dc.subjectbarley chromosomeen_US
dc.subjectsyntenic relationshipen_US
dc.subjectleaf stripeen_US
dc.subjectrice genome annotation projecten_US
dc.titleIdentification and mapping of the leaf stripe resistance gene Rdg1a in Hordeum spontaneumen_US
dc.typeJournal Articleen_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.centerCRA-GPG Genomic Research Centreen_US
cg.contributor.funderInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.projectCommunication and Documentation Information Services (CODIS)en_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.isijournalISI Journalen_US
dc.identifier.statusTimeless limited accessen_US
cg.journalTAG Theoretical and Applied Geneticsen_US

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