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dc.contributorAmin, Imranen_US
dc.contributorMansoor, Shahiden_US
dc.creatorJavaid, Shaistaen_US
dc.date2016-10-06en_US
dc.date.accessioned2017-01-11T09:24:06Z
dc.date.available2017-01-11T09:24:06Z
dc.identifierhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5052614/pdf/srep34706.pdfen_US
dc.identifierhttp://dx.doi.org/ 10.1038/srep34706en_US
dc.identifierhttps://mel.cgiar.org/reporting/download/hash/mZnA01Hten_US
dc.identifier.citationShaista Javaid, Imran Amin, Shahid Mansoor. (6/10/2016). A transgenic approach to control hemipteran insects by expressing insecticidal genes under phloem-specific promoters. United States, United States: U. S. National Library of Medicine.en_US
dc.identifier.urihttps://hdl.handle.net/20.500.11766/5489
dc.description.abstractThe first generation transgenic crops used strong constitutive promoters for transgene expression. However, tissue-specific expression is desirable for more precise targeting of transgenes. Moreover, piercing/sucking insects, which are generally resistant to insecticidal Bacillus thuringiensis (Bt) proteins, have emerged as a major pests since the introduction of transgenic crops expressing these toxins. Phloem-specific promoters isolated from Banana bunchy top virus (BBTV) were used for the expression of two insecticidal proteins, Hadronyche versuta (Blue Mountains funnel-web spider) neurotoxin (Hvt) and onion leaf lectin, in tobacco (Nicotiana tabacum). Here we demonstrate that transgenic plants expressing Hvt alone or in combination with onion leaf lectin are resistant to Phenacoccus solenopsis (cotton mealybug), Myzus persicae (green peach aphids) and Bemisia tabaci (silver leaf whitefly). The expression of both proteins under different phloem-specific promoters resulted in close to 100% mortality and provided more rapid protection than Hvt alone. Our results suggest the employment of the Hvt and onion leaf lectin transgenic constructs at the commercial level will reduce the use of chemical pesticides for control of hemipteran insect pests.en_US
dc.formatPDFen_US
dc.languageenen_US
dc.publisherU.S. National Library of Medicineen_US
dc.rightsCC-BY-NC-4.0en_US
dc.subjectWheaten_US
dc.titleA transgenic approach to control hemipteran insects by expressing insecticidal genes under phloem-specific promotersen_US
dc.typeReporten_US
cg.subject.agrovocbiotechnologyen_US
cg.subject.agrovocdisease controlen_US
cg.contributor.centerNational Institute for Biotechnology & Genetic Engineering - NIBGEen_US
cg.contributor.crpCRP on Wheat - WHEATen_US
cg.contributor.funderNot Applicableen_US
cg.contributor.project-lead-instituteUniversity of Punjab, Institute of Agricultural Sciences - UOP-IASen_US
cg.coverage.regionSouthern Asiaen_US
cg.coverage.countryPKen_US
cg.contactshahidmansoor7@gmail.comen_US
dc.identifier.statusOpen accessen_US


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