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dc.contributorAmin, Imranen_US
dc.contributorJander, Georgen_US
dc.contributorMukhtar, Zahiden_US
dc.contributorSaeed, Nasir Aen_US
dc.contributorMansoor, Shahiden_US
dc.creatorJavaid, Shaistaen_US
dc.identifier.citationShaista Javaid, Imran Amin, Georg Jander, Zahid Mukhtar, Nasir A Saeed, Shahid Mansoor. (6/10/2016). A transgenic approach to control hemipteran insects by expressing insecticidal genes under phloem-specific promoters. Scientific Reports, 6.en_US
dc.description.abstractThe first generation transgenic crops used strong constitutive promoters for transgene expression. However, tissue-specific expression is desirable for more precise targeting of transgenes. Moreover, piercing/sucking insects, which are generally resistant to insecticidal Bacillus thuringiensis (Bt) proteins, have emerged as a major pests since the introduction of transgenic crops expressing these toxins. Phloem-specific promoters isolated from Banana bunchy top virus (BBTV) were used for the expression of two insecticidal proteins, Hadronyche versuta (Blue Mountains funnel-web spider) neurotoxin (Hvt) and onion leaf lectin, in tobacco (Nicotiana tabacum). Here we demonstrate that transgenic plants expressing Hvt alone or in combination with onion leaf lectin are resistant to Phenacoccus solenopsis (cotton mealybug), Myzus persicae (green peach aphids) and Bemisia tabaci (silver leaf whitefly). The expression of both proteins under different phloem-specific promoters resulted in close to 100% mortality and provided more rapid protection than Hvt alone. Our results suggest the employment of the Hvt and onion leaf lectin transgenic constructs at the commercial level will reduce the use of chemical pesticides for control of hemipteran insect pests.en_US
dc.publisherNature Research (part of Springer Nature) (Fully open access journals)en_US
dc.sourceScientific Reports;6,(2016)en_US
dc.titleA transgenic approach to control hemipteran insects by expressing insecticidal genes under phloem-specific promotersen_US
dc.typeJournal Articleen_US
cg.subject.agrovocdisease controlen_US
cg.contributor.centerNational Institute for Biotechnology & Genetic Engineering - NIBGEen_US
cg.contributor.centerBoyce Thompson Institute - BTIen_US
cg.contributor.centerPakistan Institute of Engineering and Applied Sciences - PIEASen_US
cg.contributor.centerBoyce Thompson Instituteen_US
cg.contributor.crpCGIAR Research Program on Wheat - WHEATen_US
cg.contributor.funderUnited States Department of Agriculture - USDAen_US
cg.contributor.projectImproving Resistance to Cotton Leaf Curl Virus (CLCuV) and Supporting Cotton Best Management Practices for Small Farmersen_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.coverage.regionSouthern Asiaen_US
dc.identifier.statusOpen accessen_US

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