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dc.contributorAkhtar, Khalid Pervaizen_US
dc.contributorAkbar, Fazalen_US
dc.contributorHassan, Ishtiaqen_US
dc.contributorAmin, Imranen_US
dc.contributorSaeed, Muhammaden_US
dc.contributorMansoor, Shahiden_US
dc.creatorAli, Iftikharen_US
dc.date2016-02-01en_US
dc.date.accessioned2017-02-09T16:29:11Z
dc.date.available2017-02-09T16:29:11Z
dc.identifierhttps://mel.cgiar.org/reporting/download/hash/uuW05ZrOen_US
dc.identifier.citationIftikhar Ali, Khalid Pervaiz Akhtar, Fazal Akbar, Ishtiaq Hassan, Imran Amin, Muhammad Saeed, Shahid Mansoor. (1/2/2016). Diversity in Betasatellites Associated with Cotton Leaf Curl Disease During Source-To-Sink Movement Through a Resistant Host. The Plant Pathology Journal, 32(1), pp. 47-52.en_US
dc.identifier.urihttps://hdl.handle.net/20.500.11766/5647
dc.description.abstractCotton leaf curl is devastating disease of cotton characterized by leaf curling, vein darkening and enations. The disease symptoms are induced by DNA satellite known as Cotton leaf curl Multan betasatellite (CLCuMuB), dominant betasatellite in cotton but another betasatellite known as Chili leaf curl betasatellite (ChLCB) is also found associated with the disease. Grafting experiment was performed to determine if host plant resistance is determinant of dominant population of betasatellite in cotton (several distinct strains of CLCuMuB are associated with the disease). Infected scion of Gossypium hirsutum collected from field (the source) was grafted on G. arboreum, a diploid cotton species, resistant to the disease. A healthy scion of G. hirsutum (sink) was grafted at the top of G. arboreum to determine the movement of virus/betasatellite to upper susceptible scion of G. hirsutum. Symptoms of disease appeared in the upper scion and presence of virus/betasatellite in the upper scion was confirmed via molecular techniques, showing that virus/betasatellite was able to move to upper scion through resistant G. arboreum. However, no symptoms appeared on G. arboreum. Betasatelites were cloned and sequenced from lower scion, upper scion and G. arboreum which show that the lower scion contained both CLCuMuB and ChLCB, however only ChLCB was found in G. arboreum. The upper scion contained CLCuMuB with a deletion of 78 nucleotides (nt) in the non-coding region between A rich sequence and βC1 gene and insertion of 27 nt in the middle of βC1 ORF. This study may help in investigating molecular basis of resistance in G. arboreum.en_US
dc.formatPDFen_US
dc.languageenen_US
dc.publisherHanrimwon Publishing Companyen_US
dc.rightsCC-BY-4.0en_US
dc.sourceThe Plant Pathology Journal;32,(2016) Pagination 47,52en_US
dc.subjectbetasatelliteen_US
dc.subjectclcumuben_US
dc.subjectWheaten_US
dc.titleDiversity in Betasatellites Associated with Cotton Leaf Curl Disease During Source-To-Sink Movement Through a Resistant Hosten_US
dc.typeJournal Articleen_US
cg.subject.agrovocgossypium arboreumen_US
cg.subject.agrovocgossypium hirsutumen_US
cg.contributor.centerNational Institute for Biotechnology & Genetic Engineering - NIBGEen_US
cg.contributor.centerNuclear Institute for Agriculture and Biology, Plant Protection Division - NIAB-Faisalabaden_US
cg.contributor.centerUniversity of Swat, Center for Biotechnology and Microbiology - UoS-CBMen_US
cg.contributor.crpCRP on Wheat - WHEATen_US
cg.contributor.funderUnited States Department of Agriculture - USDAen_US
cg.contributor.projectImproving Resistance to Cotton Leaf Curl Virus (CLCuV) and Supporting Cotton Best Management Practices for Small Farmersen_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.coverage.regionSouthern Asiaen_US
cg.coverage.countryPKen_US
cg.contactkpervaiz_mbd@yahoo.comen_US
cg.identifier.doihttps://dx.doi.org/10.5423/PPJ.OA.08.2015.0160en_US
dc.identifier.statusOpen accessen_US
mel.project.openhttps://www.ars.usda.gov/research/project/?accnNo=423629en_US
mel.impact-factor1.407en_US


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