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dc.contributorJamil, Hassanen_US
dc.contributorShafiq, Muhammaden_US
dc.contributorAmin, Imranen_US
dc.contributorScheffler, Jodien_US
dc.contributorScheffler, Brianen_US
dc.contributorMansoor, Shahiden_US
dc.creatorRaza, Amiren_US
dc.date2016-04-19en_US
dc.date.accessioned2017-02-10T00:15:18Z
dc.date.available2017-02-10T00:15:18Z
dc.identifierhttp://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0153883&type=printableen_US
dc.identifierhttps://mel.cgiar.org/reporting/download/hash/lUrSy9vIen_US
dc.identifier.citationAmir Raza, Hassan Jamil, Muhammad Shafiq, Imran Amin, Jodi Scheffler, Brian Scheffler, Shahid Mansoor. (19/4/2016). RNA Interference based Approach to Down Regulate Osmoregulators of Whitefly (Bemisia tabaci): Potential Technology for the Control of Whitefly. PLoS One, 11 (4), pp. 1-13.en_US
dc.identifier.urihttps://hdl.handle.net/20.500.11766/5661
dc.description.abstractOver the past decade RNA interference (RNAi) technology has emerged as a successful tool not only for functional genomics, but in planta expression of short interfering RNAs (siRNAs) that could offer great potential for insect pest management. The diet of insects feeding exclusively on phloem sieves contains water and sugars as main components, and the uptake of the liquid food greatly depends on the osmotic pressure within the insect body. Based on this physiological mechanism, transgenic plants of Nicotiana tabacum were generated expressing double stranded RNA (dsRNA) against both aquaporin (AQP) and a sucrase gene, alpha glucosidase (AGLU). These two genes are involved in osmotic pressuremaintenance particularly in sap sucking insects, and the aim was to disrupt osmoregulationwithin the insect ultimately leading to mortality. Real time quantitative PCR (RTqPCR) was performed to assess the suppression of gene expression in Bemisia tabaci (B. tabaci) and mortality was recorded during transgenic tobacco feeding bioassays. Feeding of insects on plants expressing dsRNA significantly reduced the transcript level of the target genes in B. tabaci after six days of feeding and more than 70% mortality was observed in B. tabaci fed on transgenic plants compared to the control plants. Our data shows that downregulation of genes related to osmoregulation may find practical applications for the control of this important pest in cotton and other crops.en_US
dc.formatPDFen_US
dc.languageenen_US
dc.publisherPublic Library of Science (PLOS ONE)en_US
dc.rightsCC-BY-NC-4.0en_US
dc.sourcePLoS One;11,(2016) Pagination 1,13en_US
dc.subjectWheaten_US
dc.titleRNA Interference based Approach to Down Regulate Osmoregulators of Whitefly (Bemisia tabaci): Potential Technology for the Control of Whiteflyen_US
dc.typeJournal Articleen_US
cg.subject.agrovocbiotechnologyen_US
cg.subject.agrovocbread wheaten_US
cg.subject.agrovoccrop protectionen_US
cg.subject.agrovocdisease controlen_US
cg.contributor.centerNational Institute for Biotechnology & Genetic Engineering - NIBGEen_US
cg.contributor.centerPakistan Institute of Engineering and Applied Sciences - PIEASen_US
cg.contributor.centerUnited States Department of Agriculture, Agricultural Research Service - USDA-ARSen_US
cg.contributor.crpCGIAR Research Program on Wheat - WHEATen_US
cg.contributor.funderUnited States Department of Agriculture - USDAen_US
cg.contributor.projectImproving Resistance to Cotton Leaf Curl Virus (CLCuV) and Supporting Cotton Best Management Practices for Small Farmersen_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.coverage.regionSouthern Asiaen_US
cg.coverage.countryPKen_US
cg.contactshahidmansoor7@gmail.comen_US
cg.identifier.doihttps://dx.doi.org/10.1371/journal.pone.0153883en_US
dc.identifier.statusOpen accessen_US
mel.project.openhttps://www.ars.usda.gov/research/project/?accnNo=423629en_US
mel.impact-factor3.057en_US


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