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dc.contributorDikshit, H Ken_US
dc.contributorSingh, Dharmendraen_US
dc.contributorJain, Nivetaen_US
dc.contributorAski, Muraleedharen_US
dc.contributorSarker, Ashutoshen_US
dc.contributorSharma, T. R.en_US
dc.creatorSingh, A.K.en_US
dc.identifier.citationA. K. Singh, H K Dikshit, Dharmendra Singh, Niveta Jain, Muraleedhar Aski, Ashutosh Sarker, T. R. Sharma. (28/8/2016). Use of expressed sequence tag microsatellite markers for exploring genetic diversity in lentil and related wild species. Journal of Agricultural Science, 154 (7), pp. 1254-1269.en_US
dc.description.abstractExpressed sequence tag-simple sequence repeat (EST-SSR) markers were used to analyse genetic diversity among three Lens species. The SSR loci amplified successfully in wild species, with 94·82% transferability in Lens culinaris subsp. orientalis, 95·4% in Lens nigricans, 98·81% in L. culinaris subsp. odemensis, 94·82% in L. culinaris subsp. tomentosus and 96·55% in Lens ervoides. Ninety-nine alleles (average 3·41 alleles/locus) were detected by 29 SSR markers. Based on the unweighted pair group method with arithmetic mean cluster analysis, all the genotypes were grouped into three clusters at a similarity level of 0·30. The diversity analysis indicated no species-specific clustering of the wild and cultivated species. Wild species L. nigricans and L. culinaris subsp. ode- mensis, L. culinaris subsp. orientalis and L. ervoides were grouped in Cluster I, whereas the Mediterranean land races of L. culinaris subsp. culinaris and L. culinaris subsp. tomentosus formed a separate group in Cluster II A. Cluster II B comprised L. ervoides, L. culinaris subsp. orientalis and L. culinaris subsp. culinaris. Clusters II C, II D and II F included cultivated Indian lentil genotypes. Cluster II E comprised Indian and Mediterranean germplasm lines. Cluster II F included three early maturing germplasm lines, whereas Cluster III included only two germplasm lines. The functional annotation of SSR-containing unigenes revealed that a majority of genes were involved in an important transport-related function or were a component of metabolic pathways. A high level of polymorphism of EST-SSRs and their transferability to related wild species indicated that these markers could be used for molecular screening, map construction, comparative genomic studies and marker-assisted selection.en_US
dc.publisherCambridge University Press (CUP)en_US
dc.sourceJournal of Agricultural Science ;154,(2016) Pagination 1254,1269en_US
dc.titleUse of expressed sequence tag microsatellite markers for exploring genetic diversity in lentil and related wild speciesen_US
dc.typeJournal Articleen_US
cg.creator.idSarker, Ashutosh: 0000-0002-9074-4876en_US
cg.subject.agrovoccrop improvementen_US
cg.contributor.centerIndian Council of Agricultural Research, National Bureau of Soil Survey and Land Use Planning - ICAR- NBSS&LUPen_US
cg.contributor.centerIndian Council of Agricultural Research, Indian Agricultural Research Institute - ICAR-IARIen_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.crpCGIAR Research Program on Grain Legumes - GLen_US
cg.contributor.funderIndian Council of Agricultural Research - ICARen_US
cg.contributor.projectIndia Collaborative Program: Restricted funding for breeding for resistance to abiotic stresses in pulses & for 2017/2018 - 2017/2020 - 2020/2021en_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.coverage.regionSouthern Asiaen_US
dc.identifier.statusLimited accessen_US

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