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dc.contributorGhavami, Farhaden_US
dc.contributorHayden, Matthew Jen_US
dc.contributorWang, Yien_US
dc.contributorForrest, Kerrieen_US
dc.contributorKong, Stephanen_US
dc.contributorDizon, Rhoderissaen_US
dc.contributorMichalak de Jimenez, Monika K.en_US
dc.contributorMeinhardt, Steven W.en_US
dc.contributorMergoum, Mohameden_US
dc.contributorGu, Yong Qiangen_US
dc.contributorKianian, Shahryar F.en_US
dc.creatorBassi, Filippoen_US
dc.identifier.citationFilippo Bassi, Farhad Ghavami, Matthew J Hayden, Yi Wang, Kerrie Forrest, Stephan Kong, Rhoderissa Dizon, Monika K. Michalak de Jimenez, Steven W. Meinhardt, Mohamed Mergoum, Yong Qiang Gu, Shahryar F. Kianian. (24/2/2016). Fast-forward genetics by radiation hybrids to saturate the locus regulating nuclear–cytoplasmic compatibility in Triticum. Plant Biotechnology Journal, 14 (8), pp. 1716-1726.en_US
dc.description.abstractThe nuclear-encoded species cytoplasm specific (scs) genes control nuclear–cytoplasmic compatibility in wheat (genus Triticum). Alloplasmic cells, which have nucleus and cytoplasm derived from different species, produce vigorous and vital organisms only when the correct version of scs is present in their nucleus. In this study, bulks of in vivo radiation hybrids segregating for the scs phenotype have been genotyped by sequencing with over 1.9 million markers. The high marker saturation obtained for a critical region of chromosome 1D allowed identification of 3318 reads that mapped in close proximity of the scs. A novel in silico approach was deployed to extend these short reads to sequences of up to 70 Kb in length and identify candidate open reading frames (ORFs). Markers were developed to anchor the short contigs containing ORFs to a radiation hybrid map of 650 individuals with resolution of 288 Kb. The region containing the scs locus was narrowed to a single Bacterial Artificial Chromosome (BAC) contig of Aegilops tauschii. Its sequencing and assembly by nano-mapping allowed rapid identification of a rhomboid gene as the only ORF existing within the refined scs locus. Resequencing of this gene from multiple germplasm sources identified a single nucleotide mutation, which gives rise to a functional amino acid change. Gene expression characterization revealed that an active copy of this rhomboid exists on all homoeologous chromosomes of wheat, and depending on the specific cytoplasm each copy is preferentially expressed. Therefore, a new methodology was applied to unique genetic stocks to rapidly identify a strong candidate gene for the control of nuclear–cytoplasmic compatibility in wheat.en_US
dc.publisherWiley Open Accessen_US
dc.sourcePlant Biotechnology Journal;14,(2016) Pagination 1716,1726en_US
dc.subjectfast-forward geneticsen_US
dc.subjectspecies cytoplasm specificen_US
dc.titleFast-forward genetics by radiation hybrids to saturate the locus regulating nuclear–cytoplasmic compatibility in Triticumen_US
dc.typeJournal Articleen_US
cg.creator.idBassi, Filippo: 0000-0002-1164-5598en_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.centerNorth Dakota State University - NDSUen_US
cg.contributor.centerLa Trobe University, Centre for AgriBioscience - LATROBE - AgriBioen_US
cg.contributor.centerUnited States Department of Agriculture, Agricultural Research Service - USDA-ARSen_US
cg.contributor.centerUniversity of Georgia - UGAen_US
cg.contributor.crpCGIAR Research Program on Wheat - WHEATen_US
cg.contributor.funderInternational Maize and Wheat Improvement Center - CIMMYTen_US
cg.contributor.projectCGIAR Research Program on WHEAT - Global Alliance for Improving Food Security and the Livelihoods of the Resource-Poor in the Developing World (CRP 3.1)en_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.coverage.regionNorthern Americaen_US
dc.identifier.statusOpen accessen_US

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