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dc.creatorGhosh, Rajuen_US
dc.date2017-02-28en_US
dc.date.accessioned2017-02-28T07:38:56Z
dc.date.available2017-02-28T07:38:56Z
dc.identifierhttps://mel.cgiar.org/reporting/download/hash/kVBPYykHen_US
dc.identifier.citationRaju Ghosh. (28/2/2017). Quantification of Foc colonization through qPCR in resistant and susceptible cultivars grown under elevated CO2.en_US
dc.identifier.urihttps://hdl.handle.net/20.500.11766/6076
dc.description.abstractThe chickpea seedlings of susceptible cv. JG 62 and resistant cv. WR 315 were grown in open top chambers at different CO2 concentrations [ambient (~380 ppm) and elevated ( 550 ppm and 700 ppm). Seven day old seedlings were inoculated with Foc and samples were collected 7, 14 and 21 days of post inoculation. The sequence specific primers for quantification of Foc were designed using 18S sequences of FOC. qPCR was carried out using pure gDNA and a standard curve was generated by plotting the cycle threshold value (Ct) versus the logarithm of the concentration of each serial dilution of DNA in a 10 fold over a 7-log range from 10 to 1 × 10−4 ng/μL. The quantification of Foc in chickpea was quantified using DNA of infected plant samples through real-time PCR.en_US
dc.formatDOCXen_US
dc.languageenen_US
dc.rightsCC-BY-4.0en_US
dc.titleQuantification of Foc colonization through qPCR in resistant and susceptible cultivars grown under elevated CO2en_US
dc.typeInternal Reporten_US
cg.subject.agrovocagricultureen_US
cg.contributor.centerInternational Crops Research Institute for the Semi-Arid Tropics - ICRISATen_US
cg.contributor.crpCGIAR Research Program on Grain Legumes - GLen_US
cg.contributor.funderCGIAR System Organization - CGIARen_US
cg.coverage.regionEastern Africaen_US
cg.coverage.regionWestern Africaen_US
cg.coverage.regionSouthern Asiaen_US
cg.coverage.regionNorthern Africaen_US
cg.coverage.countryETen_US
cg.coverage.countryGHen_US
cg.coverage.countryINen_US
cg.coverage.countryMAen_US
cg.coverage.countryNGen_US
cg.contactR.Ghosh@cgiar.orgen_US
dc.identifier.statusOpen accessen_US


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