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dc.contributorTefera, Tadeleen_US
dc.contributorLemessa, Fikreen_US
dc.contributorKemal, Seid Ahmeden_US
dc.contributorFite, Tarekegnen_US
dc.contributorVillinger, Jandouween_US
dc.creatorGetaneh, Gezahegneen_US
dc.date.accessioned2021-09-30T21:36:14Z
dc.date.available2021-09-30T21:36:14Z
dc.identifierhttps://mel.cgiar.org/reporting/download/hash/4aff03afcd6060cf2b46ef9c1ebb359ben_US
dc.identifier.citationGezahegne Getaneh, Tadele Tefera, Fikre Lemessa, Seid Ahmed Kemal, Tarekegn Fite, Jandouwe Villinger. (30/9/2021). Genetic Diversity and Population Structure of Didymella rabiei Affecting Chickpea in Ethiopia. journal of Fungi, 7 (10).en_US
dc.identifier.urihttps://hdl.handle.net/20.500.11766/66176
dc.description.abstractAscochyta blight, also known as chickpea blight, which is caused by the fungal pathogen, Didymella rabiei, is an important disease affecting chickpea (Cicer arietinum L.) in many countries. We studied the genetic diversity and population structure of 96 D. rabiei isolates collected from three geographic populations in Ethiopia using simple sequence repeat (SSR) markers. We confirmed the genetic identity of 89 of the D. rabiei isolates by sequencing their rRNA internal transcribed spacer region genes. The chickpea blight pathogen isolates were genetically diverse, with a total of 51 alleles identified across 6 polymorphic SSR loci, which varied from 3 to 18 (average 8.5) alleles per SSR marker. The observed heterozygosity and expected heterozygosity ranged from 0.01 to 0.92 and 0.19 to 0.86, respectively. The mean polymorphic information content value of the D. rabiei populations was 0.58, with a mean gene diversity of 0.61 among loci. Gene flow (Nm = number of migrants) for the three populations of D. rabiei isolates ranged from 1.51 to 24.10 (average 6.2) migrants/cluster. However, the genetic variation between the D. rabiei populations was small (8%), with most of the variation occurring within populations (92%). Principal component analysis to visualize genetic variation showed that the D. rabiei isolates obtained from most of the chickpea samples formed roughly three groups on a two-dimensional coordinate plane. Similarly, the clustering of individuals into populations based on multi-locus genotypes (using Clumpak) grouped isolates into three clusters but with individual isolate admixtures. Hence, no clear geographic origin-based structuring of populations could be identified. To our knowledge, this is the first report of D. rabiei diversity in Ethiopia. Virulence studies should be conducted to develop chickpea varieties that are resistant to more aggressive pathogen populations.en_US
dc.formatPDFen_US
dc.languageenen_US
dc.publisherMDPIen_US
dc.rightsCC-BY-4.0en_US
dc.sourcejournal of Fungi;7,(2021)en_US
dc.subjectascochyta blighten_US
dc.subjectdiversityen_US
dc.subjectitsen_US
dc.titleGenetic Diversity and Population Structure of Didymella rabiei Affecting Chickpea in Ethiopiaen_US
dc.typeJournal Articleen_US
dcterms.available2021-09-30en_US
cg.creator.idKemal, Seid Ahmed: 0000-0002-1791-9369en_US
cg.subject.agrovocpopulation structureen_US
cg.subject.agrovocdidymella rabieien_US
cg.subject.agrovocchickpea blighten_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.centerInternational Center of Insect Physiology and Ecology - ICIPEen_US
cg.contributor.centerJimma University - JUen_US
cg.contributor.centerHaramaya University, College of Agriculture and Environmental Sciences - Haramaya -CoAESen_US
cg.contributor.centerEthiopian Institute of Agricultural Research, Ambo Plant Protection Research Center - EIAR - APPRCen_US
cg.contributor.funderInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.projectCommunication and Documentation Information Services (CODIS)en_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.coverage.regionEastern Africaen_US
cg.coverage.countryETen_US
cg.contactgezahegnegetaneh@gmail.comen_US
cg.identifier.doihttps://dx.doi.org/10.3390/jof7100820en_US
cg.isijournalISI Journalen_US
dc.identifier.statusOpen accessen_US
mel.impact-factor5.816en_US
cg.issn2309-608Xen_US
cg.journaljournal of Fungien_US
cg.issue10en_US
cg.volume7en_US


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