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dc.contributorWinter, P.en_US
dc.contributorWeigand, F.en_US
dc.contributorKahl, G.en_US
dc.creatorChoumane, Wafaaen_US
dc.identifier.citationWafaa Choumane, P. Winter, F. Weigand, G. Kahl. (1/7/2000). Conservation and variability of sequence-tagged microsatellite sites (STMSs) from chickpea (Cicer aerietinum L. ) within the genus Cicer. TAG Theoretical and Applied Genetics, 101, pp. 269-278.en_US
dc.description.abstractThe conservation of 90 microsatellite-flanking sequences from chickpea in 39 accessions of eight annual and 1 accession of a perennial species of the genus Cicer was investigated. All of the primer sequences successfully amplified microsatellites in related species, indicating the conservation of microsatellite-flanking sequences in chickpea's relatives. However, the degree of conservation of the primer sites varied between species depending on their known phylogenetic relationship to chickpea, ranging from 92.2% in C. reticulatum, chickpea's closest relative and potential ancestor, down to 50% for C. cuneatum. A phylogenetic tree revealed that chickpea and the other members of its crossability group were more closely related to the perennial C. anatolicum than to other annual species of the genus. Considerable variation in size and number of amplification products between and within species was observed. Sequence analysis of highly divergent amplification products proved that variation is either due to large differences in the number of microsatellite repeats or to the amplification of a locus unrelated to the one amplified from chickpea. Sequence information and bootstrapping using PAUP suggested that STMSs derived from chickpea may be efficiently and reliably used for synteny studies in chickpea's crossability group, including C. anatolicum. However, care should be taken when applying these markers to other species of the genus. Considering the data presented here and the known historical record, the age of section Monocicer including chickpea, is estimated to be about 100,000 years.en_US
dc.publisherSpringer (part of Springer Nature)en_US
dc.sourceTAG Theoretical and Applied Genetics;101,Pagination 269-278en_US
dc.subjectcicer speciesen_US
dc.subjectphylogenetic treeen_US
dc.titleConservation and variability of sequence-tagged microsatellite sites (STMSs) from chickpea (Cicer aerietinum L.) within the genus Ciceren_US
dc.typeJournal Articleen_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.centerTishreen University, Faculty of Agricultureen_US
cg.contributor.centerGoethe University-Frankfurt - GU-Frankfurten_US
cg.contributor.funderInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.projectCommunication and Documentation Information Services (CODIS)en_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.isijournalISI Journalen_US
dc.identifier.statusTimeless limited accessen_US
cg.journalTAG Theoretical and Applied Geneticsen_US

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