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dc.contributorMakris, Antoniosen_US
dc.contributorKasuko, Yamaguchi-Shinozakien_US
dc.contributorAgrawal, Shiv Kumaren_US
dc.contributorSarker, Ashutoshen_US
dc.contributorErskine, Williamen_US
dc.contributorBaum, Michaelen_US
dc.creatorKhatib, Fatehen_US
dc.identifier.citationFateh Khatib, Antonios Makris, Yamaguchi-Shinozaki Kasuko, Shiv Kumar Agrawal, Ashutosh Sarker, William Erskine, Michael Baum. (7/7/2011). Expression Of The Dreb1A Gene In Lentil (Lens Culinaris Medik. Subsp Culinaris) Transformed With The Agrobacterium System. CROP & PASTURE SCIENCE, 62 (6), pp. 488-495.en_US
dc.description.abstractUntil now three publications have reported the development of transgenic lentil plants through protocol optimisation using the gusA gene, but there are no reports of the introduction of a gene with agronomic importance. In the present study we report the introduction of the DREB1A gene into lentil to enhance drought and salinity tolerance. Decapitated embryos were immersed in Agrobacterium suspension and then co-cultivated for 4 days. Direct organogenesis was induced from the apical meristems and cotyledonary buds. Subsequently, the explants were subjected to selection in medium containing 10 mg/L phosphinothricin for nine rounds with 2-week intervals. The putative transgenic explants were micro-grafted onto non-transformed rootstocks to establish transgenic plants. The PCR results confirmed the insertion and stable inheritance of the gene of interest and bar marker gene in the plant genome. The Southern blot analysis revealed the integration of a single copy of the transgenes. T-0 plants and progeny up to T-2 generations showed complete resistance to the herbicide Basta. The DREB1A gene driven by the rd29A promoter was induced in transgenic plants by salt stress from sodium chloride solution. The total RNA was extracted and cDNA synthesised. The results showed that DREB1A mRNA was accumulated and thus the DREB1A transgene was expressed in the transgenic plants, whereas no expression was detected in the non-transformed parents.en_US
dc.publisherCSIRO Publishingen_US
dc.sourceCROP & PASTURE SCIENCE;62,(2011) Pagination 488-495en_US
dc.subjectagronomic performanceen_US
dc.subjectbar geneen_US
dc.titleExpression Of The Dreb1A Gene In Lentil (Lens Culinaris Medik. Subsp Culinaris) Transformed With The Agrobacterium Systemen_US
dc.typeJournal Articleen_US
cg.creator.idAgrawal, Shiv Kumar: 0000-0001-8407-3562en_US
cg.creator.idSarker, Ashutosh: 0000-0002-9074-4876en_US
cg.creator.idBaum, Michael: 0000-0002-8248-6088en_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.centerJapan International Research Center for Agricultural Sciences - JIRCASen_US
cg.contributor.centerInternational Centre for Advanced Mediterranean Agronomic Studies, Mediterranean Agronomic Institute of Chania - CIHEAM - IMACen_US
cg.contributor.centerUniversity of Aleppo, Faculty of Agricultureen_US
cg.contributor.crpCGIAR Research Program on Grain Legumes and Dryland Cereals - GLDCen_US
cg.contributor.funderGlobal Centre of Excellence for Dry Land Scienceen_US
cg.contributor.projectCommunication and Documentation Information Services (CODIS)en_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.isijournalISI Journalen_US
dc.identifier.statusTimeless limited accessen_US

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