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dc.contributorSen, Senjutien_US
dc.contributorGhosh, Prithwien_US
dc.contributorSengupta, Aninditaen_US
dc.contributorBasu, Debabrataen_US
dc.contributorDas, Sampaen_US
dc.creatorChakrabarti, Jaydeeben_US
dc.identifier.citationJaydeeb Chakrabarti, Senjuti Sen, Prithwi Ghosh, Anindita Sengupta, Debabrata Basu, Sampa Das. (30/6/2016). Homologous promoter derived constitutive and chloroplast targeted expression of synthetic cry1Ac in transgenic chickpea confers resistance against Helicoverpa armigera. Plant Cell, Tissue and Organ Culture (PCTOC), 125 (3), pp. 521-535.en_US
dc.description.abstractThe insecticidal crystal protein derived from gram positive soil bacterium Bacillus thuringiensis plays an important role in controlling lepidopteran infestation. The present study seeks to protect chickpea plants from Helicoverpa armigera infestation by over expressing cry1Ac. Homologous Ubiquitin and RuBisCO small subunit (rbcS) promoters were used to transcribe cry1Ac in transgenic chickpea both constitutively and in a tissue specific manner through Agrobacterium mediated transformation of chickpea var. ICCV89314. Expressed Cry1Ac was specifically targeted to the chloroplast rich tissues using transit peptide sequence. After monitoring transgene integration by Southern hybridization, transgenic chickpea lines were further analyzed by western blot, ELISA and insect bioassay. Expression of cry1Ac in chickpea under the control of above two promoters conferred a high level of protection against pod borer infestation, where chloroplast targeting system was found to be more efficient in controlling this particular devastating lepidopteran pest.en_US
dc.publisherSpringer Verlag (Germany)en_US
dc.sourcePlant Cell, Tissue and Organ Culture (PCTOC);125,(2016) Pagination 521,535en_US
dc.subjectchloroplast transit peptideen_US
dc.subjectchloroplast targeted expressionen_US
dc.subjectrubisco small subunit promoteren_US
dc.subjecttransgenic chickpeaen_US
dc.subjectubiquitin promoteren_US
dc.titleHomologous promoter derived constitutive and chloroplast targeted expression of synthetic cry1Ac in transgenic chickpea confers resistance against Helicoverpa armigeraen_US
dc.typeJournal Articleen_US
cg.contributor.centerBose Instituteen_US
cg.contributor.centerInternational Crops Research Institute for the Semi-Arid Tropics - ICRISATen_US
cg.contributor.crpCGIAR Research Program on Grain Legumes - GLen_US
cg.contributor.funderNot Applicableen_US
cg.coverage.regionSouthern Asiaen_US
dc.identifier.statusLimited accessen_US

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