Molecular Breeding for introgression of fatty acid desaturase mutant alleles (ahFAD2A and ahFAD2B) enhances oil quality in high and low oil containing peanut genotypes
Author(s)
Citation
Pasupuleti Janila, Gyan Mishra, T. Radhakrishnan, K. L. Dobariya, N Manivannan, R. P. Vasanthi, Pawan Khera, Rajeev Varshney, Surendra Manohar, Nagesh Patne, Yaduru Shasidhar, Manda Sriswathi, Manish K. Vishwakarma, Manish K Pandey, TV Murali. (Accepted on 28/8/2015). Molecular Breeding for introgression of fatty acid desaturase mutant alleles (ahFAD2A and ahFAD2B) enhances oil quality in high and low oil containing peanut genotypes. Plant Science.
Abstract
High oleate peanuts have two marketable benefits, health benefits to consumers and extended shelf life
of peanut products. Two mutant alleles present on linkage group a09 (ahFAD2A) and b09 (ahFAD2B) control
composition of three major fatty acids, oleic, linoleic and palmitic acids which together determine
peanut oil quality. In conventional breeding, selection for fatty acid composition is delayed to advanced
generations. However by using DNA markers, breeders can reject large number of plants in early generations
and therefore can optimize time and resources. Here,two approaches of molecular breeding namely
marker-assisted backcrossing (MABC) and marker-assisted selection (MAS) were employed to transfer
two FAD2 mutant alleles from SunOleic 95R into the genetic background of ICGV 06110, ICGV 06142
and ICGV 06420. In summary, 82 MABC and 387 MAS derived introgression lines (ILs) were developed
using DNA markers with elevated oleic acid varying from 62 to 83%. Oleic acid increased by 0.5–1.1 folds,
with concomitant reduction of linoleic acid by 0.4–1.0 folds and palmitic acid by 0.1–0.6 folds among ILs
compared to recurrent parents. Finally, high oleate ILs, 27 with high oil (53–58%), and 28 ILs with low oil
content (42–50%) were selected that may be released for cultivation upon further evaluation
Collections
- Agricultural Research Knowledge [11119]
Author(s) ORCID(s)
Janila, Pasupuletihttps://orcid.org/0000-0003-2583-9630
Pandey, Manish Khttps://orcid.org/0000-0002-4101-6530
Murali, TVhttps://orcid.org/0000-0001-5775-0329
Subject(s)
AGROVOC Keywords
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