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dc.creatorAkem, Chrysantusen_US
dc.identifier.citationChrysantus Akem. (26/11/2010). Ascochyta blight of chickpea: present status and future priorities. International Journal of Pest Management, 45 (2), pp. 131-137.en_US
dc.description.abstractA size-selected genomic library comprising 280,000 colonies and representing approximate to 18% of the chickpea genome, was screened for (GA)(n), (GAA)(n) and (TAA)(n) microsatellite-containing clones, of which 389 were sequenced. The majority (similar to 75%) contained perfect repeats; interrupted, interrupted compound and compound repeats were only present in 6%-9% of cases. (TAA)-microsatellites contained the longest repeats, with unit numbers from 9 to 131. For 218 loci primers could be designed and used for the detection of microsatellite length polymorphisms in six chickpea breeding cultivars, as well as in C. reticulatum and C. echinospermum, wild, intercrossable relatives of chickpea. A total of 174 primer pairs gave interpretable banding patterns, 137 (79%) of which revealed at least two alleles on native polyacrylamide gels. A total of 120 sequence-tagged microsatellite site (STMS) markers were genetically mapped in 90 recombinant lines from an inter-species cross between C. reticulatum and the chickpea cultivar ICC 4958. Markers could be arranged in 11 linkage groups (at a LOD score of 4) covering 613 cM. Clustering as well as random distribution of loci was observed. Segregation of 46 markers (39%) deviated significantly (P greater than or equal to 0.05) from the expected 1:1 ratio, The majority of these loci (73%) were located in three distinct regions of the genome. The present STMS marker map represents the most advanced co-dominant DNA marker map of the chickpea genome.en_US
dc.publisherTaylor and Francisen_US
dc.sourceInternational Journal of Pest Management;45,(2010) Pagination 131-137en_US
dc.subjectgenome mappingen_US
dc.subjectsequence-tagged microsatellite markersen_US
dc.subjectprimer sequencesen_US
dc.titleAscochyta blight of chickpea: present status and future prioritiesen_US
dc.typeJournal Articleen_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.funderInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.projectCommunication and Documentation Information Services (CODIS)en_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.isijournalISI Journalen_US
dc.identifier.statusTimeless limited accessen_US
cg.journalInternational Journal of Pest Managementen_US

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