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dc.contributorSajjalaguddam, Ramgopal Raoen_US
dc.contributorKumar, K. Vijay Krishnaen_US
dc.contributorVarshney, Rajeeven_US
dc.contributorSudini, Hari kishanen_US
dc.creatorJacob, Simien_US
dc.identifier.citationSimi Jacob, Ramgopal Rao Sajjalaguddam, K. Vijay Krishna Kumar, Rajeev Varshney, Hari kishan Sudini. (31/3/2016). Assessing the prospects of Streptomyces sp. RP1A-12 in managing groundnut stem rot disease caused by Sclerotium rolfsii Sacc. Journal of General Plant Pathology, 82 (2), pp. 96-104.en_US
dc.description.abstractStem rot of groundnut caused by the soilborne pathogen Sclerotium rolfsii can cause significant yield losses. Biological control of stem rot using actinomycetes is a viable alternative to existing fungicidal management. Though actinomycetes are prolific antibiotic producers, reports pertaining to their use in groundnut disease management are limited. Here, actinomycetes were isolated from groundnut rhizospheric soils and screened for antagonism against S. rolfsii through a dual culture assay. Culture filtrates and crude extracts of the potential candidates were screened further for extracellular antifungal activity and characterized for biocontrol and plant-growth-promoting traits. A promising candidate was tested under greenhouse conditions as whole organism as well as crude extracts. Isolate RP1A-12 exhibited high antagonism against S. rolfsii in dual culture assay (69 % inhibition), culture filtrate assay (78–100 % inhibition at various concentrations) and crude extract assay (100 % inhibition with 1 % crude extracts). Moreover, germination of sclerotia of the test pathogen was inhibited with 1 % crude extracts. Strain RP1A-12 produced hydrogen cyanide, lipase, siderophores and indole acetic acid. Oxalic acid production by S. rolfsii was also inhibited by crude extracts of RP1A-12. In greenhouse studies, RP1A-12 reduced stem rot severity. Overall, our results suggest that isolate RP1A-12 has potential biocontrol capabilities against stem rot pathogen. Molecular characterization based on 16S rRNA gene sequencing of RP1A-12 identified it as a species of Streptomyces, closely related to S. flocculusen_US
dc.publisherSpringer Verlag (Germany)en_US
dc.sourceJournal of General Plant Pathology;82,(2016) Pagination 96-104en_US
dc.subjectstreptomyces sp.en_US
dc.subjectstem roten_US
dc.subjectantifungal metabolitesen_US
dc.titleAssessing the prospects of Streptomyces sp. RP1A-12 in managing groundnut stem rot disease caused by Sclerotium rolfsii Saccen_US
dc.typeJournal Articleen_US
cg.contributor.centerInternational Crops Research Institute for the Semi-Arid Tropics - ICRISATen_US
cg.contributor.centerJawaharlal Nehru Technological University Hyderabad - JNTUHen_US
cg.contributor.centerSreenidhi Institute of Science and Technology - SNISTen_US
cg.contributor.crpCGIAR Research Program on Grain Legumes - GLen_US
cg.contributor.funderNot Applicableen_US
cg.contributor.project-lead-instituteInternational Crops Research Institute for the Semi-Arid Tropics - ICRISATen_US
cg.coverage.regionSouthern Asiaen_US
cg.isijournalISI Journalen_US
dc.identifier.statusLimited accessen_US
cg.journalJournal of General Plant Pathologyen_US

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