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dc.contributorBhatnagar - Mathur, Poojaen_US
dc.contributorReddy, P. Sudhakaren_US
dc.contributorCindhuri, Katamreddy Srien_US
dc.contributorGanesh, Adusumalli Sivajien_US
dc.contributorSharma, Kiranen_US
dc.creatorReddy, Dumbala Srinivasen_US
dc.date.accessioned2017-04-12T07:37:44Z
dc.date.available2017-04-12T07:37:44Z
dc.identifierhttp://oar.icrisat.org/id/eprint/9325en_US
dc.identifierhttps://mel.cgiar.org/reporting/download/hash/zW14nkIEen_US
dc.identifier.citationDumbala Srinivas Reddy, Pooja Bhatnagar - Mathur, P. Sudhakar Reddy, Katamreddy Sri Cindhuri, Adusumalli Sivaji Ganesh, Kiran Sharma. (10/2/2016). Identification and Validation of Reference Genes and Their Impact on Normalized Gene Expression Studies across Cultivated and Wild Cicer Species. PLoS ONE, 11 (2).en_US
dc.identifier.urihttps://hdl.handle.net/20.500.11766/6735
dc.description.abstractQuantitative Real-Time PCR (qPCR) is a preferred and reliable method for accurate quantification of gene expression to understand precise gene functions. A total of 25 candidate reference genes including traditional and new generation reference genes were selected and evaluated in a diverse set of chickpea samples. The samples used in this study included nine chickpea genotypes (Cicer spp.) comprising of cultivated and wild species, six abiotic stress treatments (drought, salinity, high vapor pressure deficit, abscisic acid, cold and heat shock), and five diverse tissues (leaf, root, flower, seedlings and seed). The geNorm, NormFinder and RefFinder algorithms used to identify stably expressed genes in four sample sets revealed stable expression of UCP and G6PD genes across genotypes, while TIP41 and CAC were highly stable under abiotic stress conditions. While PP2A and ABCT genes were ranked as best for different tissues, ABCT, UCP and CAC were most stable across all samples. This study demonstrated the usefulness of new generation reference genes for more accurate qPCR based gene expression quantification in cultivated as well as wild chickpea species. Validation of the best reference genes was carried out by studying their impact on normalization of aquaporin genes PIP1;4 and TIP3;1, in three contrasting chickpea genotypes under high vapor pressure deficit (VPD) treatment. The chickpea TIP3;1 gene got significantly up regulated under high VPD conditions with higher relative expression in the drought susceptible genotype, confirming the suitability of the selected reference genes for expression analysis. This is the first comprehensive study on the stability of the new generation reference genes for qPCR studies in chickpea across species, different tissues and abiotic stresses.en_US
dc.formatPDFen_US
dc.languageenen_US
dc.publisherPublic Library of Scienceen_US
dc.rightsCC-BY-4.0en_US
dc.sourcePLoS ONE;11,(2016)en_US
dc.subjectplant materialsen_US
dc.subjectgenome sequenceen_US
dc.subjectgene expression studiesen_US
dc.subjectstress treatmentsen_US
dc.titleIdentification and Validation of Reference Genes and Their Impact on Normalized Gene Expression Studies across Cultivated and Wild Cicer Speciesen_US
dc.typeJournal Articleen_US
dcterms.available2016-02-10en_US
cg.creator.idBhatnagar - Mathur, Pooja: 0000-0002-4150-8287en_US
cg.subject.agrovocgene expressionen_US
cg.subject.agrovocfunctional genomicsen_US
cg.subject.agrovocchickpeasen_US
cg.contributor.centerInternational Crops Research Institute for the Semi-Arid Tropics - ICRISATen_US
cg.contributor.crpCGIAR Research Program on Grain Legumes - GLen_US
cg.contributor.funderCGIAR System Organization - CGIARen_US
cg.coverage.regionEastern Africaen_US
cg.coverage.regionWestern Africaen_US
cg.coverage.regionSouthern Asiaen_US
cg.coverage.regionNorthern Africaen_US
cg.coverage.countryETen_US
cg.coverage.countryGHen_US
cg.coverage.countryINen_US
cg.coverage.countryMAen_US
cg.coverage.countryNGen_US
cg.contactP.BHATNAGAR@CGIAR.ORGen_US
cg.identifier.doihttps://dx.doi.org/10.1371/journal.pone.0148451en_US
cg.isijournalISI journalen_US
dc.identifier.statusOpen accessen_US
mel.impact-factor2.766en_US
cg.issn1932-6203en_US
cg.journalPLoS ONEen_US
cg.issue2en_US
cg.volume11en_US


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