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dc.contributorWaeyenberge, Lievenen_US
dc.contributorViaene, Nicoleen_US
dc.contributorDababat, Abdelfattahen_US
dc.contributorNicol, Julieen_US
dc.contributorOgbonnaya, Francis Chuksen_US
dc.contributorMoens, Mauriceen_US
dc.creatorToumi, Fatehen_US
dc.identifier.citationFateh Toumi, Lieven Waeyenberge, Nicole Viaene, Abdelfattah Dababat, Julie Nicol, Francis Chuks Ogbonnaya, Maurice Moens. (26/5/2015). Development of qPCR assays for quantitative detection of Heterodera avenae and H. latipons. European Journal of Plant Pathology, 143 (2), pp. 305-316.en_US
dc.description.abstractTwelve Heterodera species are considered of major economic significance in cereals, of which Heterodera avenae, H. latipons and H. filipjevi are the most important. Precise identification and quantification of these nematodes are necessary to develop effective integrated pest control. This study reports on the use of the mitochondrial cytochrome oxidase subunit 1 (COI) gene to develop qPCR assays that could be used for the identification and quantification of H. avenae and H. latipons. Two qPCR primer sets, each comprising two primers and a probe, were designed for each of both species. After optimization, the qPCR assays using a single second-stage juvenile (J2) were able to identify and quantify H. avenae and H. latipons. Their specificity was confirmed by the lack of amplification of J2 of 14 other Heterodera species. A qPCR using DNA extracted from 120 J2 + eggs of H. avenae and H. latipons resulted in steady Ct-values (Ct = 22.33 +/- 0.1 and Ct = 21.83 +/- 0.12, respectively). Dilution series of DNA extracted from 120 J2 + eggs of the two species were made. The assays for both species resulted in a standard curve showing a highly significant linearity between the Ct-values and the dilution rates (R-2 = 0.99; slope = -3.03 and R-2 = 0.99; slope = -3.28 for H. avenae and H. latipons, respectively). The two qPCR assays provide a sensitive and valid tool for rapid detection and quantification of the two species whether they occur alone or in mixtures with other species.en_US
dc.publisherSpringer (part of Springer Nature)en_US
dc.sourceEuropean Journal of Plant Pathology;143,(2015) Pagination 305-316en_US
dc.subjectcytochrome oxidase subunit 1 (coi) geneen_US
dc.subjecth. avenaeen_US
dc.subjecth. latiponsen_US
dc.titleDevelopment of qPCR assays for quantitative detection of Heterodera avenae and H. latiponsen_US
dc.typeJournal Articleen_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.centerInternational Maize and Wheat Improvement Center - CIMMYTen_US
cg.contributor.centerGrains Research and Development Corporation - GRDCen_US
cg.contributor.centerInstitute for Agricultural and Fisheries Research - ILVOen_US
cg.contributor.centerGhent University, Faculty of Bioscience Engineering - GU - BWen_US
cg.contributor.funderMonsanto, Beachell-Borlaug International Scholars Program**en_US
cg.contributor.projectCommunication and Documentation Information Services (CODIS)en_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.isijournalISI Journalen_US
dc.identifier.statusTimeless limited accessen_US
cg.journalEuropean Journal of Plant Pathologyen_US

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