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dc.contributorSatoh, Koujien_US
dc.contributorKondoh, Hiroakien_US
dc.contributorAsano, Takayukien_US
dc.contributorHosaka, Aenien_US
dc.contributorVenuprasad, Ramiahen_US
dc.contributorSerraj, Rachiden_US
dc.contributorKumar, Arvinden_US
dc.contributorLeung, Heien_US
dc.contributorKikuchi, Shoshien_US
dc.creatorMoumeni, Alien_US
dc.identifier.citationAli Moumeni, Kouji Satoh, Hiroaki Kondoh, Takayuki Asano, Aeni Hosaka, Ramiah Venuprasad, Rachid Serraj, Arvind Kumar, Hei Leung, Shoshi Kikuchi. (2/12/2011). Comparative analysis of root transcriptome profiles of two pairs of drought-tolerant and susceptible rice near-isogenic lines under different drought stress. BMC Plant Biology, 11.en_US
dc.description.abstractBackground: Plant roots are important organs to uptake soil water and nutrients, perceiving and transducing of soil water deficit signals to shoot. The current knowledge of drought stress transcriptomes in rice are mostly relying on comparative studies of diverse genetic background under drought. A more reliable approach is to use near-isogenic lines (NILs) with a common genetic background but contrasting levels of resistance to drought stress under initial exposure to water deficit. Here, we examined two pairs of NILs in IR64 background with contrasting drought tolerance. We obtained gene expression profile in roots of rice NILs under different levels of drought stress help to identify genes and mechanisms involved in drought stress. Results: Global gene expression analysis showed that about 55% of genes differentially expressed in roots of rice in response to drought stress treatments. The number of differentially expressed genes (DEGs) increased in NILs as the level of water deficits, increased from mild to severe condition, suggesting that more genes were affected by increasing drought stress. Gene onthology (GO) test and biological pathway analysis indicated that activated genes in the drought tolerant NILs IR77298-14-1-2-B-10 and IR77298-5-6-B-18 were mostly involved in secondary metabolism, amino acid metabolism, response to stimulus, defence response, transcription and signal transduction, and down-regulated genes were involved in photosynthesis and cell wall growth. We also observed gibberellic acid (GA) and auxin crosstalk modulating lateral root formation in the tolerant NILs. Conclusions: Transcriptome analysis on two pairs of NILs with a common genetic background (similar to 97%) showed distinctive differences in gene expression profiles and could be effective to unravel genes involved in drought tolerance. In comparison with the moderately tolerant NIL IR77298-5-6-B-18 and other susceptible NILs, the tolerant NIL IR77298-14-1-2-B-10 showed a greater number of DEGs for cell growth, hormone biosynthesis, cellular transports, amino acid metabolism, signalling, transcription factors and carbohydrate metabolism in response to drought stress treatments. Thus, different mechanisms are achieving tolerance in the two tolerant lines.en_US
dc.publisherBioMed Centralen_US
dc.sourceBMC Plant Biology;11,(2011)en_US
dc.subjectabiotic stressesen_US
dc.subjectcellulose synthase-likeen_US
dc.subjectoryza-sativa len_US
dc.subjectwater-deficit stressen_US
dc.subjectresponsive genesen_US
dc.titleComparative analysis of root transcriptome profiles of two pairs of drought-tolerant and susceptible rice near-isogenic lines under different drought stressen_US
dc.typeJournal Articleen_US
cg.subject.agrovocabiotic stressen_US
cg.subject.agrovocupland riceen_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.centerAfrica Rice Center - AfricaRiceen_US
cg.contributor.centerInternational Rice Research Institute - IRRIen_US
cg.contributor.centerNational Institute of Agrobiological Sciences** - NIAS Japanen_US
cg.contributor.funderGeneration Challenge Programme (GCP)en_US
cg.contributor.projectCommunication and Documentation Information Services (CODIS)en_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.isijournalISI Journalen_US
dc.identifier.statusOpen accessen_US
cg.journalBMC Plant Biologyen_US

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