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dc.contributorZhang, Weiboen_US
dc.contributorWang, Shanen_US
dc.contributorGe, Lingen_US
dc.contributorCao, Xiukaien_US
dc.contributorWang, Shanheen_US
dc.contributorYuan, Zehuen_US
dc.contributorLv, Xiaoyangen_US
dc.contributorGetachew, Tesfayeen_US
dc.contributorMwacharo, Joramen_US
dc.contributorHaile, Aynalemen_US
dc.contributorSun, Weien_US
dc.creatorHe, Mingliangen_US
dc.identifier.citationMingliang He, Weibo Zhang, Shan Wang, Ling Ge, Xiukai Cao, Shanhe Wang, Zehu Yuan, Xiaoyang Lv, Tesfaye Getachew, Joram Mwacharo, Aynalem Haile, Wei Sun. (16/3/2022). MicroRNA-181a Regulates the Proliferation and Differentiation of Hu Sheep Skeletal Muscle Satellite Cells and Targets the YAP1 Gene. Genes, 13 (3).en_US
dc.description.abstractMicroRNA (miRNA) is of great importance to muscle growth and development, including the regulation of the proliferation and differentiation of skeletal muscle satellite cells (SMSCs). In our research group’s previous study, we found that miR-181a is differentially expressed in the longissimus dorsi muscle of Hu sheep at different stages. We speculated that miR-181a may participate in the growth and development process of Hu sheep. To understand the mechanism of miR-181a regulating the growth and development of Hu sheep skeletal muscle, we extracted skeletal muscle satellite cells from the longissimus dorsi muscle of 3-month-old Hu sheep fetuses and performed a series of experiments. Our results showed that miR-181a suppressed SMSCs’ proliferation using QRT-PCR, Western blot, CCK-8, EDU, and Flow cytometry cycle tests. In addition, QRT-PCR, Western blot, and immunofluorescence indicated that miR-181a facilitated the differentiation of SMSCs. Then, we used dual-luciferase reporter gene detection, QRT-PCR, and Western blot to find that the Yes1-related transcription regulator (YAP1) is the target gene of miR-181a. Our study supplies a research basis for understanding the regulation mechanism of miR-181a on the growth of Hu sheep skeletal muscle.en_US
dc.subjecthu sheepen_US
dc.subjectno povertyen_US
dc.subjectpoverty reduction, livelihoods and jobsen_US
dc.subjectsatellite cellen_US
dc.titleMicroRNA-181a Regulates the Proliferation and Differentiation of Hu Sheep Skeletal Muscle Satellite Cells and Targets the YAP1 Geneen_US
dc.typeJournal Articleen_US
cg.creator.idGetachew, Tesfaye: 0000-0002-0544-6314en_US
cg.creator.idMwacharo, Joram: 0000-0001-6981-8140en_US
cg.creator.idHaile, Aynalem: 0000-0001-5914-0487en_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.centerYangzhou University, College of Animal Science and Technology - YZU - CASTen_US
cg.contributor.centerYangzhou University, MOE Joint International Research Laboratory of Agriculture and Agri-Product Safety - YZU - MOE JIRLAAPSen_US
cg.contributor.crpResilient Agrifood Systems - RAFSen_US
cg.contributor.funderNational Natural Science Foundation of China - NSFCen_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.isijournalISI Journalen_US
dc.identifier.statusOpen accessen_US
cg.subject.sdgSDG 1 - No povertyen_US
cg.subject.actionAreaResilient Agrifood Systemsen_US
cg.subject.impactAreaPoverty reduction, livelihoods and jobsen_US
cg.contributor.initiativeSustainable Animal Productivityen_US

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