Next-generation sequencing for identification of candidate genes for Fusarium wilt and sterility mosaic disease in pigeonpea (Cajanus cajan)
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Sinha Pallavi, Vikas Singh, Aamir Khan, Rajeev Varshney, Lakshmanan Krishnamurty, Sarwar Azam, Pazhamala Lekha, Rachit Saxena. (Accepted on 6/1/2016). Next-generation sequencing for identification of candidate genes for Fusarium wilt and sterility mosaic disease in pigeonpea (Cajanus cajan). Frontiers in Plant Science.
Pigeonpea is a resilient crop, which is relatively more drought tolerant than many other legume crops. To understand the molecular mechanisms of this unique feature of pigeonpea, 51 genes were selected using the Hidden Markov Models (HMM) those codes for proteins having close similarity to universal stress protein domain. Validation of these genes was conducted on three pigeonpea genotypes (ICPL 151, ICPL 8755, and ICPL 227) having different levels of drought tolerance. Gene expression analysis using qRT-PCR revealed 6, 8, and 18 genes to be ≥ 2-fold differentially expressed in ICPL 151, ICPL 8755, and ICPL 227, respectively. A total of 10 differentially expressed genes showed ≥ 2-fold up-regulation in the more drought tolerant genotype, which encoded four different classes of proteins. These include plant U-box protein (four genes), universal stress protein A-like protein (four genes), cation/H(+) antiporter protein (one gene) and an uncharacterized protein (one gene). Genes C.cajan_29830 and C.cajan_33874 belonging to uspA, were found signiﬁcantly expressed in all the three genotypes with ≥ 2-fold expression variations. Expression proﬁling of these two genes on the four other legume crops revealed their speciﬁc role in pigeonpea. Therefore, these genes seem to be promising candidates for conferring drought tolerance speciﬁcally to pigeonpea.