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dc.contributorBajaj, Deepaken_US
dc.contributorDas, Shouviken_US
dc.contributorkumar, vinoden_US
dc.contributorGowda, C. L. L.en_US
dc.contributorSharma, Shivalien_US
dc.contributorTyagi, Akhilesh K.en_US
dc.contributorParida, Swarup K.en_US
dc.creatorUpadhyaya, Hari D.en_US
dc.date.accessioned2017-04-20T10:42:48Z
dc.date.available2017-04-20T10:42:48Z
dc.identifierhttps://mel.cgiar.org/reporting/download/hash/pS3mhRinen_US
dc.identifier.citationHari D. Upadhyaya, Deepak Bajaj, Shouvik Das, vinod kumar, C. L. L. Gowda, Shivali Sharma, Akhilesh K. Tyagi, Swarup K. Parida. (11/4/2016). Genetic dissection of seed-iron and zinc concentrations in chickpea. Scientific Reports, 6: 24050.en_US
dc.identifier.urihttps://hdl.handle.net/20.500.11766/6842
dc.description.abstractThe SNP-based high-resolution QTL mapping mapped eight major genomic regions harbouring robust QTLs governing seed-Fe and Zn concentrations (39.4% combined phenotypic variation explained/PVE) on six chromosomes of an intra-specific high-density genetic linkage map (1.56 cM map-density). 24620 SNPs discovered from genome-wide GBS (genotyping-by-sequencing) and 13 known cloned Fe and Zn contents-related chickpea gene-orthologs were genotyped in a structured population of 92 sequenced desi and kabuli accessions. The large-scale 16591 SNP genotyping- and phenotyping-based GWAS (genome-wide association study) identified 16 genomic loci/genes associated (29% combined PVE) with seed-Fe and Zn concentrations. Of these, 11 trait-associated SNPs in the genes linked tightly with eight QTLs were validated by QTL mapping. The seed-specific expression, including pronounced differential-regulation of 16 trait-associated genes particularly in accessions/mapping individuals with contrasting level of seed-Fe and Zn contents was apparent. Collectively, the aforementioned rapid integrated genomic strategy led to delineate novel functional non-synonymous and regulatory SNP allelic-variants from 16 known/candidate genes, including three strong trait-associated genes (encoding late embryogenesis abundant and yellow stripe-like 1 protein, and vacuolar protein sorting-associated protein) and eight major QTLs regulating seed-Fe and Zn concentrations in chickpea. These essential inputs thus have potential to be deployed in marker-assisted genetic enhancement for developing nutritionally-rich iron/zinc-biofortified chickpea cultivars.en_US
dc.formatPDFen_US
dc.languageenen_US
dc.publisherNature Research (part of Springer Nature) (Fully open access journals)en_US
dc.rightsCC-BY-4.0en_US
dc.sourceScientific Reports; 6:24050,(2016)en_US
dc.subjectgeneticen_US
dc.subjectgenome widea ssociation studiesen_US
dc.titleGenetic dissection of seed-iron and zinc concentrations in chickpeaen_US
dc.typeJournal Articleen_US
dcterms.available2016-04-11en_US
cg.subject.agrovocqtl mappingen_US
cg.subject.agrovocgenetic markersen_US
cg.subject.agrovocgeneticsen_US
cg.subject.agrovocchickpeasen_US
cg.subject.agrovocChickpeaen_US
cg.contributor.centerInternational Crops Research Institute for the Semi-Arid Tropics - ICRISATen_US
cg.contributor.centerNational Institute of Plant Genome Research - NIPGRen_US
cg.contributor.crpCGIAR Research Program on Grain Legumes - GLen_US
cg.contributor.funderCGIAR System Organization - CGIARen_US
cg.contributor.project-lead-instituteInternational Crops Research Institute for the Semi-Arid Tropics - ICRISATen_US
cg.coverage.regionSouthern Asiaen_US
cg.coverage.countryINen_US
cg.contactH.UPADHYAYA@CGIAR.ORGen_US
cg.identifier.doihttps://dx.doi.org/10.1038/srep24050en_US
cg.isijournalISI journalen_US
dc.identifier.statusOpen accessen_US
mel.impact-factor4.122en_US
cg.issn2045-2322en_US
cg.journalScientific Reportsen_US
cg.volume6:24050en_US


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