Show simple item record

dc.contributorLi, Beien_US
dc.contributorPandey, Manish Ken_US
dc.contributorWu, Yanshanen_US
dc.contributorLei, Yongen_US
dc.contributorYan, Liyingen_US
dc.contributorDai, Xiaofengen_US
dc.contributorJiang, Huifangen_US
dc.contributorZhang, Junchengen_US
dc.contributorWei, Guoen_US
dc.contributorVarshney, Rajeeven_US
dc.contributorLiao, Boshouen_US
dc.creatorwan, liyunen_US
dc.date2016-10-31en_US
dc.date.accessioned2017-04-26T21:15:38Z
dc.date.available2017-04-26T21:15:38Z
dc.identifierhttp://oar.icrisat.org/id/eprint/9838en_US
dc.identifierhttps://mel.cgiar.org/reporting/download/hash/w2dtVnfXen_US
dc.identifier.citationLiyun wan, Bei Li, Manish K Pandey, Yanshan Wu, Yong Lei, Liying Yan, Xiaofeng Dai, Huifang Jiang, Juncheng Zhang, Guo Wei, Rajeev Varshney, Boshou Liao. (31/10/2016). Transcriptome Analysis of a New Peanut Seed Coat Mutant for the Physiological Regulatory Mechanism Involved in Seed Coat Cracking and Pigmentation. Frontiers in Plant Science, 7 (1491), pp. 1-15.en_US
dc.identifier.urihttps://hdl.handle.net/20.500.11766/6892
dc.description.abstractSeed-coatcrackingandundesirablecolorofseedcoathighlyaffectsexternalappearanceandcommercialvalueofpeanuts(ArachishypogaeaL.).Withanobjectivetofindgeneticsolutiontotheaboveproblems,apeanutmutantwithcrackingandbrowncoloredseedcoat(testa)wasidentifiedfromanEMStreatedmutantpopulationanddesignatedas“peanutseedcoatcrackandbrowncolormutantline(pscb).”Theseedcoatweightofthemutantwasalmosttwiceofthewildtype,andthegerminationtimewassignificantlyshorterthanwildtype.Further,themutanthadlowerleveloflignin,anthocyanin,proanthocyanidincontent,andhighlyincreasedlevelofmelanincontentascomparedtowildtype.UsingRNA-Seq,weexaminedtheseedcoattranscriptomeinthreestagesofseeddevelopmentinthewildtypeandthepscbmutant.TheRNA-Seqanalysisrevealedpresenceofhighlydifferentiallyexpressedphenylpropanoidandflavonoidpathwaygenesinallthethreeseeddevelopmentstges,especiallyat40daysafterflowering(DAF40).Also,theexpressionofpolyphenoloxidasesandperoxidasewerefoundtobeactivatedsignificantlyespeciallyinthelateseeddevelopmentalstage.Thegenome-widecomparativestudyoftheexpressionprofilesrevealed62differentiallyexpressedgenescommonacrossallthethreestages.Byanalyzingtheexpressionpatternsandthesequencesofthecommondifferentiallyexpressedgenesofthethreestages,threecandidategenesnamelyc36498_g1(CCoAOMT1),c40902_g2(kinesin),andc33560_g1(MYB3)wereidentifiedresponsibleforseed-coatcrackingandbrowncolorphenotype.Therefore,thisstudynotonlyprovidedcandidategenesbutalsoprovidedgreaterinsightsandmoleculargeneticcontrolofpeanutseed-coatcrackingandcolorvariation.Theinformationgeneratedinthisstudywillfacilitatefurtheridentificationofcausalgeneanddiagnosticmarkersforbreedingimprovedpeanutvrietieswithsmoothanddesirableseedcoatcolor.en_US
dc.formatPDFen_US
dc.languageenen_US
dc.publisherFrontiers Mediaen_US
dc.rightsCC-BY-NC-4.0en_US
dc.sourceFrontiers in Plant Science;7 (1491),(2016) Pagination 1,15en_US
dc.subjectrna-seqen_US
dc.subjectpeanut (arachishypogaea)en_US
dc.subjectseed-coatcrackingen_US
dc.subjectflavonoidpathwayen_US
dc.subjectPeanuten_US
dc.titleTranscriptome Analysis of a New Peanut Seed Coat Mutant for the Physiological Regulatory Mechanism Involved in Seed Coat Cracking and Pigmentationen_US
dc.typeJournal Articleen_US
cg.creator.idPandey, Manish K: 0000-0002-4101-6530en_US
cg.creator.ID-typeORCIDen_US
cg.subject.agrovocagricultureen_US
cg.subject.agrovocpigmentationen_US
cg.contributor.centerChinese Academy of Agricultural Sciences, Oil Crops Research Institute - CAAS-OCRIen_US
cg.contributor.centerInternational Crops Research Institute for the Semi-Arid Tropics - ICRISATen_US
cg.contributor.centerChinese Academy of Agricultural Sciences, Institiute of Food Sciencea and Technology - CAAS-FOODCAASen_US
cg.contributor.crpCGIAR Research Program on Grain Legumes - GLen_US
cg.contributor.funderNot Applicableen_US
cg.coverage.regionEastern Asiaen_US
cg.coverage.regionSouthern Asiaen_US
cg.coverage.countryCNen_US
cg.coverage.countryINen_US
cg.contactlboshou@hotmail.comen_US
cg.identifier.doihttps://dx.doi.org/10.3389/fpls.2016.01491en_US
dc.identifier.statusOpen accessen_US
mel.impact-factor4.495en_US


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record


DSpace software copyright © 2002-2016  DuraSpace
Disclaimer:
MELSpace content providers and partners accept no liability to any consequence resulting from use of the content or data made available in this repository. Users of this content assume full responsibility for compliance with all relevant national or international regulations and legislation.
Theme by 
Atmire NV