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dc.contributorZheng, Yunen_US
dc.contributorKudapa, Hima binduen_US
dc.contributorJagadeeswaran, Guruen_US
dc.contributorHivrale, Vandanaen_US
dc.contributorVarshney, Rajeeven_US
dc.contributorSunkar, Ramanjuluen_US
dc.creatorSrivastava, Sangeetaen_US
dc.identifier.citationSangeeta Srivastava, Yun Zheng, Hima bindu Kudapa, Guru Jagadeeswaran, Vandana Hivrale, Rajeev Varshney, Ramanjulu Sunkar. (26/6/2015). Plant Science Volume 235, June 2015, Pages 46–57 Cover image High throughput sequencing of small RNA component of leaves and inflorescence revealed conserved and novel miRNAs as well as phasiRNA loci in chickpea. Plant Science, 235, pp. 46-57.en_US
dc.description.abstractAmong legumes, chickpea (Cicer arietinum L.) is the second most important crop after soybean. MicroRNAs (miRNAs) play important roles by regulating target gene expression importantfor plant development and tolerance to stress conditions. Additionally, recently discovered phased siRNAs (phasiRNAs), a new class of small RNAs, are abundantly produced in legumes. Nevertheless, little is known about these regulatory molecules in chickpea. The small RNA population was sequenced from leaves and flowers of chickpea to identify conserved and novel miRNAs as well as phasiRNAs/phasiRNA loci. Bioinformatics analysis revealed 157 miRNA loci for the 96 highly conserved and known miRNA homologs belonging to 38 miRNA families in chickpea. Furthermore, 20 novel miRNAs belonging to 17 miRNA families were identified. Sequence analysis revealed approximately 60 phasiRNA loci. Potential target genes likely to be regulated by these miRNAs were predicted and some were confirmed by modified 5 RACE assay. Predicted targets are mostly transcription factors that might be important for developmental processes, and others include superoxide dismutases, plantacyanin, laccases and F-box proteins that could participate in stress responses and protein degradation. Overall, this study provides an inventory of miRNA–target gene interactions for chickpea, useful for the comparative analysis of small RNAs among legumes.en_US
dc.sourcePlant Science;235,(2015) Pagination 46-57en_US
dc.subjectposttranscriptional gene regulationsen_US
dc.titlePlant Science Volume 235, June 2015, Pages 46–57 Cover image High throughput sequencing of small RNA component of leaves and inflorescence revealed conserved and novel miRNAs as well as phasiRNA loci in chickpeaen_US
dc.typeJournal Articleen_US
cg.contributor.centerInternational Crops Research Institute for the Semi-Arid Tropics - ICRISATen_US
cg.contributor.centerOklahoma State University - OSUen_US
cg.contributor.centerKunming University of Science and Technologyen_US
cg.contributor.crpCGIAR Research Program on Grain Legumes - GLen_US
cg.contributor.funderNot Applicableen_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.isijournalISI Journalen_US
dc.identifier.statusOpen accessen_US
cg.journalPlant Scienceen_US

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