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dc.contributorUpadhyaya, Hari D.en_US
dc.contributorKhan, Yusufen_US
dc.contributorDas, Shouviken_US
dc.contributorBadoni, Saurabhen_US
dc.contributorShree, Tanimaen_US
dc.contributorKumar, Vinoden_US
dc.contributorTripathi, Shaileshen_US
dc.contributorGowda, C. L. L.en_US
dc.contributorSingh, Subeen_US
dc.contributorSharma, Shivalien_US
dc.contributorTyagi, Akhilesh K.en_US
dc.contributorChattopadhyay, Debasisen_US
dc.contributorParida, Swarup K.en_US
dc.creatorBajaj, Deepaken_US
dc.date2015-07-17en_US
dc.date.accessioned2017-05-14T23:19:02Z
dc.date.available2017-05-14T23:19:02Z
dc.identifierhttp://oar.icrisat.org/id/eprint/8892en_US
dc.identifierhttps://mel.cgiar.org/reporting/download/hash/OBiAAbgfen_US
dc.identifier.citationDeepak Bajaj, Hari D. Upadhyaya, Yusuf Khan, Shouvik Das, Saurabh Badoni, Tanima Shree, Vinod Kumar, Shailesh Tripathi, C. L. L. Gowda, Sube Singh, Shivali Sharma, Akhilesh K. Tyagi, Debasis Chattopadhyay, Swarup K. Parida. (17/7/2015). A combinatorial approach of comprehensive QTL-based comparative genome mapping and transcript profiling identified a seed weight-regulating candidate gene in chickpea. Scientific Reports, 5 (9264), pp. 1-14.en_US
dc.identifier.urihttps://hdl.handle.net/20.500.11766/6982
dc.description.abstractHigh experimental validation/genotyping success rate (94–96%) and intra-specific polymorphic potential (82–96%) of 1536 SNP and 472 SSR markers showing in silico polymorphism between desi ICC 4958 and kabuli ICC 12968 chickpea was obtained in a 190 mapping population (ICC 4958 3 ICC 12968) and 92 diverse desi and kabuli genotypes. A high-density 2001 marker-based intra-specific genetic linkage map comprising of eight LGs constructed is comparatively much saturated (mean map-density: 0.94 cM) in contrast to existing intra-specific genetic maps in chickpea. Fifteen robust QTLs (PVE: 8.8–25.8% with LOD: 7.0–13.8) associated with pod and seed number/plant (PN and SN) and 100 seed weight (SW) were identified and mapped on 10 major genomic regions of eight LGs. One of 126.8 kb major genomic region harbouring a strong SW-associated robust QTL (Caq’SW1.1: 169.1–171.3 cM) has been delineated by integrating high-resolution QTL mapping with comprehensive marker-based comparative genome mapping and differential expression profiling. This identified one potential regulatory SNP (G/A) in the cis-acting element of candidate ERF (ethylene responsive factor) TF (transcription factor) gene governing seed weight in chickpea. The functionally relevant molecular tags identified have potential to be utilized for marker-assisted genetic improvement of chickpeaen_US
dc.formatPDFen_US
dc.languageenen_US
dc.publisherNature Publishing Groupen_US
dc.rightsCC-BY-NC-4.0en_US
dc.sourceScientific Reports;5,(2015) Pagination 1,14en_US
dc.subjectqtl mappingen_US
dc.subjectgenome mappingen_US
dc.subjectChickpeaen_US
dc.titleA combinatorial approach of comprehensive QTL-based comparative genome mapping and transcript profiling identified a seed weight-regulating candidate gene in chickpeaen_US
dc.typeJournal Articleen_US
cg.subject.agrovocplant genetic resourcesen_US
cg.subject.agrovocgenotypingen_US
cg.subject.agrovocchickpeasen_US
cg.contributor.centerNational Institute of Plant Genome Research - NIPGRen_US
cg.contributor.centerInternational Crops Research Institute for the Semi-Arid Tropics - ICRISATen_US
cg.contributor.centerIndian Council of Agricultural Research, Indian Agricultural Research Institute - ICAR-IARIen_US
cg.contributor.crpCGIAR Research Program on Grain Legumes - GLen_US
cg.contributor.funderNot Applicableen_US
cg.coverage.regionGlobalen_US
cg.contactswarup@nipgr.ac.inen_US
cg.identifier.doihttps://dx.doi.org/10.1038/srep09264en_US
dc.identifier.statusOpen accessen_US
mel.impact-factor5.228en_US


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