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dc.contributorUpadhyaya, Hari D.en_US
dc.contributorBajaj, Deepaken_US
dc.contributorKujur, Aliceen_US
dc.contributorBadoni, Saurabhen_US
dc.contributorNarnoliya, laXMIen_US
dc.contributorKumar, Vinoden_US
dc.contributorTripathi, Shaileshen_US
dc.contributorGowda, CL Laxmipathien_US
dc.contributorSharma, Shivalien_US
dc.contributorSingh, Subeen_US
dc.contributorTyagi, Akhilesh K.en_US
dc.contributorParida, Swarup K.en_US
dc.creatorDas, Shouviken_US
dc.date.accessioned2017-05-15T15:13:44Z
dc.date.available2017-05-15T15:13:44Z
dc.identifierhttp://oar.icrisat.org/id/eprint/8694en_US
dc.identifierhttps://mel.cgiar.org/reporting/download/hash/wjl4B1DPen_US
dc.identifier.citationShouvik Das, Hari D. Upadhyaya, Deepak Bajaj, Alice Kujur, Saurabh Badoni, laXMI Narnoliya, Vinod Kumar, Shailesh Tripathi, CL Laxmipathi Gowda, Shivali Sharma, Sube Singh, Akhilesh K. Tyagi, Swarup K. Parida. (3/5/2015). Deploying QTL-seq for rapid delineation of a potential candidate gene underlying major trait-associated QTL in chickpea. DNA Research, 22 (2), pp. 1-11.en_US
dc.identifier.urihttps://hdl.handle.net/20.500.11766/6994
dc.description.abstractA rapid high-resolution genome-wide strategy for molecular mapping of major QTL(s)/gene(s) regulating important agronomic traits is vital for in-depth dissection of complex quantitative traits and genetic enhancement in chickpea. The present study for the first time employed a NGS-based whole-genomeQTL-seq strategy to identify one major genomic region harbouring a robust 100- seed weight QT Lusinganintra-specific 221 chickpea mapping population (desicv.ICC7184×desicv.ICC 15061). The QTL-seq-derived major SW QTL (CaqSW1.1) was further validated by single-nucleotide polymorphism (SNP) and simple sequence repeat (SSR) marker-based traditional QTL mapping (47.6% R2 at higher LOD >19). This reflects the reliability and efficacy of QTL-seq as a strategy for rapid genome-wide scanning and fine mapping of major trait regulatory QTLs in chickpea. The use of QTL-seq and classical QTL mapping in combination narrowed down the 1.37 Mb (comprising 177genes) major SWQTL (CaqSW1.1) regionintoa 35 kb genomic intervalondesi chickpea chromosome 1 containing six genes. One coding SNP (G/A)-carrying constitutive photomorphogenic 9 (COP9) signalo some complex subunit (CSN8) gene of the see xhibited seed-specific expression, including pronounced differential up-/down-regulation in low and high seed weight mapping parents and homo zygous individuals duringseed development.The coding SNP mined in this potential seed weight- governing candidate CSN8 genewas found to be present exclusively in all cultivated species/ genotypes, but notin any wild species/genotypes of primary, secondary and tertiary gene pools.This indicates the effect of strong artificial and/or natural selection pressure on target SW locus during chickpea domestication. The proposed QTL-seq-driven integrated genome-wide strategy has potential to delineate major candidate gene(s) harbouring a robust trait regulatory QTL rapidly with optimal use of resources. This will further assist us to extrapolate the molecular mechanism underlying complex quantitative traits at a genome-wide scale leading to fast-paced marker-assisted genetic improvement in diverse crop plants, including chickpea.en_US
dc.formatPDFen_US
dc.languageenen_US
dc.publisherOxford University Press (OUP)en_US
dc.rightsCC-BY-NC-4.0en_US
dc.sourceDNA Research;22,(2015) Pagination 1-11en_US
dc.subjectqtl-seqen_US
dc.subjectngsen_US
dc.subjectqtlen_US
dc.titleDeploying QTL-seq for rapid delineation of a potential candidate gene underlying major trait-associated QTL in chickpeaen_US
dc.typeJournal Articleen_US
dcterms.available2015-05-03en_US
dcterms.extent1-11en_US
cg.subject.agrovocplant genetic resourcesen_US
cg.subject.agrovocsnpen_US
cg.subject.agrovocChickpeaen_US
cg.contributor.centerInternational Crops Research Institute for the Semi-Arid Tropics - ICRISATen_US
cg.contributor.centerIndian Council of Agricultural Research, Indian Agricultural Research Institute - ICAR-IARIen_US
cg.contributor.centerGlobal Research-for-development Support Ventures - GRSVen_US
cg.contributor.centerNational Institute of Plant Genome Research - NIPGRen_US
cg.contributor.crpCGIAR Research Program on Grain Legumes - GLen_US
cg.contributor.funderNot Applicableen_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contactswarup@nipgr.ac.inen_US
cg.identifier.doihttps://dx.doi.org/10.1093/dnares/dsv004en_US
cg.isijournalISI Journalen_US
dc.identifier.statusOpen accessen_US
mel.impact-factor5.267en_US
cg.issn1340-2838en_US
cg.journalDNA Researchen_US
cg.issue2en_US
cg.volume22en_US


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