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dc.contributorFarah, J.en_US
dc.contributorMoussally, S.en_US
dc.contributorEl-Shamaa, Khaleden_US
dc.contributorAlmer, K.en_US
dc.contributorKhierallah, H.en_US
dc.contributorUdupa, Sripada M.en_US
dc.contributorLababidi, Sameren_US
dc.contributorMalek, Joel A.en_US
dc.contributorAaouine, M.en_US
dc.contributorBaum, Michaelen_US
dc.creatorHamwieh, Aladdinen_US
dc.identifier.citationAladdin Hamwieh, J. Farah, S. Moussally, Khaled El-Shamaa, K. Almer, H. Khierallah, Sripada M. Udupa, Samer Lababidi, Joel A. Malek, M. Aaouine, Michael Baum. (26/11/2010). Development of 1000 Microsatellite Markers across the Date Palm (Phoenix dactylifera L. ) Genome. Acta Horticulturae, 882, pp. 269-277.en_US
dc.description.abstractDate Palm is a major environmental and economic factor in arid climates in many countries around the world. Microsatellite markers have been proven to be very powerful in plant genome analysis because they are locus-specific, codominant, highly polymorphic and highly reproducible. In date palm only few microsatellite markers have been developed so far. Recently, the Cornell Medical College in Qatar issued a draft assembly of the date palm genome (‘Khalas’) generated by whole genome shotgun next generation DNA sequencing. In this paper, we analyzed the microsatellite motifs across the date palm genome. The results indicated that the most abundant type of microsatellite repeats are dinucleotide repeats (52442 motifs) followed by trinucleotide (28503 motifs) and pentanucleotide repeats (12873 motifs). The frequencies of tetra-nucleotide and hexa-nucleotide repeats were less across the genome (5555 and 5810 motifs, respectively). The most common type of dinucleotide repeat was GA (48.7%) followed by AT (37%). Out of 28645 trinucleotide repeats, TAA and GAA repeats were the most abundant repeats (28.1 and 27.1%) respectively. More than 1090 new microsatellite markers could be designed. The primary test for 50 primer pairs revealed that 28 (56%) were functional and 19 (38%) yielded polymorphic PCR products. We wish that the results of our study will be a starting point for researchers making use of the markers for genetic mapping and diversity analysis of date palm.en_US
dc.publisherInternational Society for Horticultural Science (ISHS)en_US
dc.sourceActa Horticulturae ;882,(2010) Pagination 269,277en_US
dc.subjectp. dactyliferaen_US
dc.subjectmicrosatellite markeren_US
dc.subjectadh geneen_US
dc.titleDevelopment of 1000 Microsatellite Markers across the Date Palm (Phoenix dactylifera L.) Genomeen_US
dc.typeJournal Articleen_US
cg.creator.idHamwieh, Aladdin: 0000-0001-6060-5560en_US
cg.creator.idEl-Shamaa, Khaled: 0000-0002-7668-3798en_US
cg.creator.idUdupa, Sripada M.: 0000-0003-4225-7843en_US
cg.creator.idBaum, Michael: 0000-0002-8248-6088en_US
cg.subject.agrovocsimple sequence repeatsen_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.centerUniversity of Aleppoen_US
cg.contributor.centerQatar universityen_US
cg.contributor.centerUniversity of Baghdad, College of Agricultureen_US
cg.contributor.centerWeill Cornell Medical College - Qatar - WCM-Qen_US
cg.contributor.funderInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.projectCommunication and Documentation Information Services (CODIS)en_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.coverage.regionWestern Asiaen_US
dc.identifier.statusOpen accessen_US

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