Large-scale microsatellite development in grasspea ( Lathyrus sativus L.), an orphan legume of the arid areas
Impact factor: 3.964 (Year: 2014)
MetadataShow full item record
Tao Wang, Junye Jiang, Marina Burlyaeva, Jinguo Hu, Clarice J Coyne, Shiv Kumar Agrawal, Bob Redden, Xuelian Sun, Fang Wang, Jianwu Chang, Xiaopeng Hao, Jianping Guan, Xuxiao Zong. (17/3/2014). Large-scale microsatellite development in grasspea ( Lathyrus sativus L. ), an orphan legume of the arid areas. BMC Plant Biology, 14: 65.
Background Grasspea (Lathyrus sativus L., 2n = 14), a member of the family Leguminosae, holds great agronomic potential as grain and forage legume crop in the arid areas for its superb resilience to abiotic stresses such as drought, flood and salinity. The crop could not make much progress through conventional breeding in the past, and there are hardly any detailed molecular biology studies due to paucity of reliable molecular markers representative of the entire genome. Results Using the 454 FLX Titanium pyrosequencing technique, 651,827 simple sequence repeat (SSR) loci were identified and 50,144 nonredundant primer pairs were successfully designed, of which 288 were randomly selected for validation among 23 L. sativus and one L. cicera accessions of diverse provenance. 74 were polymorphic, 70 monomorphic, and 144 with no PCR product. The number of observed alleles ranged from two to five, the observed heterozygosity from 0 to 0.9545, and Shannon’s information index ranged from 0.1013 to 1.0980, respectively. The dendrogram constructed by using unweighted pair group method with arithmetic mean (UPGMA) based on Nei's genetic distance, showed obvious distinctions and understandable relationships among the 24 accessions. Conclusions The large number of SSR primer pairs developed in this study would make a significant contribution to genomics enabled improvement of grasspea.
Agrawal, Shiv Kumarhttps://orcid.org/0000-0001-8407-3562
Showing items related by title, author, creator and subject.
Author(s)Deng, Juan; Xie, Xing-Long; wang, Dong-Feng; Zhao, Chao; Lv, Feng-Hua; Li, Xin; Yang, Ji; Yu, Jia-Lin; Shen, Min; Gao, Lei; Yang, Jing-Quan; Liu, Ming-Jun; Li, Wen-Rong; Wang, Yu-Tao; Wang, Feng; Li, Jin-Quan; Hehua, Eer; Liu, Yong-Gang; Shen, Zhi-Qiang; Ren, Yan-Ling; Liu, Guang-Jian; Chen, Ze-Hui; Gorkhali, Neena A; E Rushdi, Hossam; Salehian-Dehkordi, Hosein; Esmailizadeh, Ali; Nosrati, Maryam; R. Paiva, Samuel; R Caetano, Alexandre; Štěpánek, Ondřej; Olsaker, Ingrid; Weimann, Christina; Erhardt, Georg; Curik, Ino; Kantanen, Juha; Mwacharo, Joram; Hanotte, Olivier; W Bruford, Michael; Ciani, Elena; Periasamy, Kathiravan; Amills, Marcel; A Lenstra, Johannes; Jianlin, Han; Zhang, Hong-Ping; Li, Li; Li, Meng-HuaDate: 2020-10-19Status: Open accessThe domestication and subsequent global dispersal of livestock are crucial events in human history, but the migratory episodes during the history of livestock remain poorly documented [1, 2, 3]. Here, we first developed a ...
Title: Historical Introgression from Wild Relatives Enhanced Climatic Adaptation and Resistance to Pneumonia in Sheep Author(s)Cao, Yin-Hong; Xu, Song-Song; Shen, Min; Chen, Ze-Hui; Gao, Lei; Lv, Feng-Hua; Xie, Xing-Long; Wang, Xin-Hua; Yang, Hua; Liu, Chang-Bin; Zhou, Ping; Wan, Peng-Cheng; Zhang, Yun-Sheng; Yang, Jing-Quan; Pi, Wen-Hui; Hehua, Eer; P. Berry, Donagh; Barbato, Mario; Esmailizadeh, Ali; Nosrati, Maryam; Salehian-Dehkordi, Hosein; Dehghani-Qanatqestani, Mostafa; Dotsev, Arsen V.; Deniskova, Tatiana E.; Zinovieva, Natalia A.; Brem, Gottfried; Stepanek, Ondrej; Ciani, Elena; Weimann, Christina; Erhardt, Georg; Mwacharo, Joram; Ahbara, Abulgasim; Jianlin, Han; Hanotte, Olivier; Miller, Joshua M.; Sim, Zijian; Coltman, David W.; Kantanen, Juha; W Bruford, Michael; A Lenstra, Johannes; Kijas, James W.; Li, Meng-HuaDate: 2021-03-01Status: Open accessHow animals, particularly livestock, adapt to various climates and environments over short evolutionary time is of fundamental biological interest. Further, understanding the genetic mechanisms of adaptation in indigenous ...
Title: The genome of cultivated peanut provides insight into legume karyotypes, polyploid evolution and crop domestication Author(s)Zhuang, Weijian; Chen, Hua; Yang, Meng; Wang, Jianping; Pandey, Manish K; Zhang, Chong; Chang, Wen-Chi; Zhang, Liangsheng; Zhang, Xingtan; Tang, Ronghua; Vanika, Garg; Wang, Xingjun; Tang, Haibao; Chow, Chi-Nga; Wang, Jinpeng; Deng, Ye; Wang, Depeng; Khan, Aamir; Yang, Qiang; Cai, Tiecheng; Bajaj, Prasad; Wu, Kangcheng; Guo, Baozhu; Zhang, Xinyou; Li, Jingjing; Liang, Fan; Hu, Jiang; Liao, Boshou; Liu, Shengyi; Chitikineni, Annapurna; Yan, Hansong; Zheng, Yixiong; Shan, Shihua; Liu, Qinzheng; Xie, Dongyang; Wang, Zhenyi; Khan, Shahid; Ali, Niaz; Zhao, Chuanzhi; Li, Xinguo; Luo, Ziliang; Zhang, Shubiao; Zhuang, Ruirong; Peng, Ze; Wang, Shuaiyan; Mamadou, Gandeka; Zhuang, Yuhui; Zhao, Zifan; Yu, Weichang; Xiong, Faqian; Quan, Weipeng; Yuan, Mei; Li, Yu; Zou, Huasong; Xia, Han; Zha, Li; Fan, Junpeng; Yu, Jigao; Xie, Wenping; Yuan, Jiaqing; Chen, Kun; Zhao, Shanshan; Chu, Wenting; Chen, Yuting; Sun, Pengchuan; Meng, Fanbo; Zhuo, Tao; Zhao, Yuhao; Li, Chunjuan; He, Guohao; Zhao, Yongli; Wang, Congcong; Kavi Kishor, Polavarapu B.; Pan, Rong-Long; Paterson, Andrew H.; wang, xiyin; Ming, Ray; Varshney, RajeevDate: 2019-05-01Status: Open accessHigh oil and protein content make tetraploid peanut a leading oil and food legume. Here we report a high-quality peanut genome sequence, comprising 2.54 Gb with 20 pseudomolecules and 83,709 protein-coding gene models. We ...