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dc.contributorSharma, N.en_US
dc.contributorAgrawal, Shiv Kumaren_US
dc.creatorBarpete, Surendraen_US
dc.identifier.citationSurendra Barpete, N. Sharma, Shiv Kumar Agrawal. (27/8/2014). Assessment of somaclonal variation and stability in in vitro regenerated grass pea plants using SDS-PAGE. Legume Research, 37 (4), pp. 345-352.en_US
dc.description.abstractTissue culture may be one of the possible sources of variation for crop improvement. To assess variation and stability in regenerated plants, shoots were regenerated from the callus derived from axillary explants of 11 grasspea genotypes, and their shoot protein profiles were compared with those of seed cultured plants. The highest response of callus induction (87%) was observed when 2.0 mg/l2,4-dichlorophenoxyacetic acid(2,4-D) and 0.25 mg/l BAP were supplemented in Murashige and Skoog’s (MS) medium. -naphthaleneacetic acid(NAA) and 6-benzylaminopurine(BAP) showed variable redifferentiation response along with callus formation. The MS medium supplemented with 0.5 mg/l BAP showed better multiplication and elongation of shoots.Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) analysis showed a unique protein band of 43 kDa in both tissue and seed cultured plants of Pusa 24. Polypeptide banding pattern of regenerated plants from 11 grasspea genotypes did not deviate from the banding pattern of parental seed protein. Similarity coefficient values ranged from 0.37 to 0.85 with a mean of 0.43 among the 55 genotypic combinations. Comparison of protein bands between calli raised regenerated shoots and parental seeds revealed the absence of somaclonal variation in regenerated plants, suggesting that the regeneration protocol used in the present study can be used for genomics enabled improvement in grasspea without the risk of additional variation or instability.en_US
dc.publisherAgricultural Research Communication Centre- ARCCen_US
dc.sourceLegume Research;37,(2014) Pagination 345,352en_US
dc.subjectaxillary shootsen_US
dc.subjectin-vitro cultureen_US
dc.subjectGrass pea (Lathyrus sativus)en_US
dc.titleAssessment of somaclonal variation and stability in in vitro regenerated grass pea plants using SDS-PAGEen_US
dc.typeJournal Articleen_US
cg.creator.idBarpete, Surendra: 0000-0002-5547-8488en_US
cg.creator.idAgrawal, Shiv Kumar: 0000-0001-8407-3562en_US
cg.subject.agrovocsomaclonal variationen_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.centerBarkatullah University - BU Bhopalen_US
cg.contributor.crpCGIAR Research Program on Grain Legumes - GLen_US
cg.contributor.funderInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.projectCommunication and Documentation Information Services (CODIS)en_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.coverage.regionSouthern Asiaen_US
dc.identifier.statusOpen accessen_US

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