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dc.contributorGharbi, Mohameden_US
dc.contributorMhadhbi, Moezen_US
dc.contributorMabrouk, M.en_US
dc.contributorAyari, Boutheınaen_US
dc.contributorNasfi, Inesen_US
dc.contributorJedidi, Mohameden_US
dc.contributorSassi, Limamen_US
dc.contributorRekik, Mouraden_US
dc.contributorDarghouth, Mohamed Azizen_US
dc.creatorRjaibi, Mohameden_US
dc.identifier.citationMohamed Rjaibi, Mohamed Gharbi, Moez Mhadhbi, M. Mabrouk, Boutheına Ayari, Ines Nasfi, Mohamed Jedidi, Limam Sassi, Mourad Rekik, Mohamed Aziz Darghouth. (22/5/2014). Prevalence of piroplasms in small ruminants in North-West Tunisia and the first genetic characterisation of Babesia ovis in Africa. Parasite, 21 (23).en_US
dc.description.abstractIn this study, the prevalence of piroplasms in sheep and goats was assessed with Giemsa-stained blood smear examination, PCR and nested PCR-restriction fragment length polymorphism (RFLP) to identify Babesia and Theileria species, respectively, in 338 small ruminants (172 sheep and 166 goats) from three sites in North-West Tunisia during the 2011 summer season. The overall infection prevalence of piroplasms in Giemsa-stained blood smears was 3.2% (11/338), with a parasitaemia ranging from 0.01 to 0.05%. PCR detected two species, namely Babesia ovis (in sheep and goats) and Theileria ovis (in sheep), with an overall prevalence of 16.3%. The molecular prevalence of B. ovis was significantly higher in sheep than in goats (17.4% and 9%, respectively, p = 0.034). The same trend was observed for T. ovis in sheep and goats (5.8% and 0%, respectively, p = 0.004). Comparison of the partial sequences of the 18S rRNA gene revealed 100% similarity amongst Babesia from sheep and goats. The single Theileria sequence in this study showed 100% similarity to T. ovis. A high similarity with all the blasted genotypes was reported for Theileria and Babesia sequences. This is the first molecular detection of B. ovis and genetic characterisation of small ruminants’ piroplasms in Africa.en_US
dc.publisherEDP Sciencesen_US
dc.titlePrevalence of piroplasms in small ruminants in North-West Tunisia and the first genetic characterisation of Babesia ovis in Africaen_US
dc.typeJournal Articleen_US
cg.creator.idRekik, Mourad: 0000-0001-7455-2017en_US
cg.subject.agrovocsmall ruminantsen_US
cg.subject.agrovocbabesia ovisen_US
cg.subject.agrovoctheileria ovisen_US
cg.contributor.centerManouba University, National School of Veterinary Medicine of Sidi Thabet - ENMVen_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.crpCGIAR Research Program on Dryland Systems - DSen_US
cg.contributor.funderInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.projectCommunication and Documentation Information Services (CODIS)en_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.coverage.regionNorthern Africaen_US
dc.identifier.statusOpen accessen_US

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