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dc.contributorDakora, Felix D.en_US
dc.creatorSriswathi, Mandaen_US
dc.identifier.citationManda Sriswathi, Felix D. Dakora. (23/9/2015). Assessing the genetic diversity of 48 groundnut (Arachis hypogaea L. ) genotypes in the Guinea savanna agro-ecology of Ghana, using microsatellite-based markers. African Journal of Biotechnology, 14 (32), pp. 2484-2493.en_US
dc.description.abstractGroundnut (Arachis hypogaea L.) is the most important grain legume in Ghana. However, its production is constrained by a myriad of biotic and abiotic stresses which necessitate the development and use of superior varieties for increased yield. Germplasm characterisation both at the phenotypic and molecular level is important in all plant breeding programs. The aim of this study was to characterise selected advanced breeding groundnut lines with different phenotypic attributes at the molecular level using simple sequence repeats (SSR) markers in Ghana. A total of 53 SSR markers were screened and 25 were found to be polymorphic with an average polymorphic information content (PIC) value of 0.57. Of the 48 groundnut genotypes studied, 67% showed very close relationship (~100% similarity) with one or more genotypes among themselves. In fact, there were 14 instances where two to three genotypes within the same sub-cluster exhibited 100% similarity even though they displayed different phenotypic attributes. The remaining 33% of the groundnut genotypes were distant from each other and could therefore serve as effective parental material for future work. In this study, the SSR-based markers were found to be quite discriminatory in discerning variations between and among groundnut lines even where the level of variation was low. Microsatellite-based markers therefore represent a useful tool for dissecting genetic variations in cultivated crops, especially groundnuten_US
dc.publisherAcademic Journalsen_US
dc.sourceAfrican Journal of Biotechnology,;14,(2015) Pagination 2484,2493en_US
dc.subjectphenotypic traitsen_US
dc.subjectpcr amplificationen_US
dc.subjectsimple sequence repeats (ssr) markersen_US
dc.subjectpolymorphic information contenten_US
dc.subjectjaccard’s similarity coefficen_US
dc.subjectdna extractionen_US
dc.titleAssessing the genetic diversity of 48 groundnut (Arachis hypogaea L.) genotypes in the Guinea savanna agro-ecology of Ghana, using microsatellite-based markersen_US
dc.typeJournal Articleen_US
cg.subject.agrovocplant genetic resourcesen_US
cg.contributor.centerInternational Crops Research Institute for the Semi-Arid Tropics - ICRISATen_US
cg.contributor.centerTshwane University of Technology - TUTen_US
cg.contributor.crpCGIAR Research Program on Grain Legumes - GLen_US
cg.contributor.funderNot Applicableen_US
cg.coverage.regionWestern Africaen_US
cg.isijournalISI journalen_US
dc.identifier.statusLimited accessen_US
cg.journalAfrican Journal of Biotechnology,en_US

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